Cloning and expression of a thermostable pullulanase gene from Thermotoga maritima MSB8 in Bacillus subtilis WB600

被引：0

作者：

Su, Zhe ^{[1
]}

Lu, Fu-Ping ^{[1
]}

Gao, Qiang ^{[1
]}

Liu, Xiao-Guang ^{[1
]}

Wang, Bin-Zhe ^{[1
]}

Niu, Tao ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Biotechnol, Minist Educ, Key Lab Ind Microbiol, Tianjin 300457, Peoples R China

来源：

2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010) | 2010年

关键词：

Pullulanase; Thermotoga maritima MSB8; Bacillussubtilis WB600; pWB980; Enzymatic properties;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The pulA gene enconding a thermostable pullulanase of 844 amino acids was cloned by PCR amplification from the genomic DNA of Thermotoga maritima MSB8. We successfully expressed the pulA gene in a protease-deficient strain Bacillus subtilis WB600, and the expressed pullulanase was secreted into the culture broth. Preliminary results showed that the pullulanase exhibited optimal activity at 90 degrees C , and was stable at the temperature range from 30 to 80 degrees C for 30 min. The stable pH range for this pullulanase was from 5.5 to 7.0, with an optimum at pH 6.0.

引用

页数：4

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