Electrochemistry and biosensing of glucose oxidase immobilized on Pt-dispersed mesoporous carbon

被引：27
|
作者：
You, Chunping [1 ,2 ,3 ]
Li, Xiang [1 ,2 ]
Zhang, Song [1 ,2 ]
Kong, Jilie [1 ,2 ]
Zhao, Dongyuan [1 ,2 ]
Liu, Baohong [1 ,2 ]
机构：
[1] Fudan Univ, Dept Chem, Shanghai Key Lab Mol Catalysis & Novel Mat, Shanghai 200433, Peoples R China
[2] Fudan Univ, Inst Adv Mat & Biomed Sci, Shanghai 200433, Peoples R China
[3] Bright Dairy & Food Co Ltd, Ctr Technol, Shanghai 200436, Peoples R China
来源：
MICROCHIMICA ACTA | 2009年 / 167卷 / 1-2期
关键词：
Glucose oxidase; Biosensor; Electron transfer; Mesoporous carbon; Nanoparticles; DIRECT ELECTRON-TRANSFER; PLATINUM NANOPARTICLES; HYDROGEN-PEROXIDE; PASTE ELECTRODES; NANOTUBES; LAYER; FABRICATION; MATRIX; FILM; BIOCATALYSIS;
D O I：
10.1007/s00604-009-0228-1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for immobilizing proteins in a carbon mesoporous material (CMM) containing platinum nanoparticles (Pt-NPs) is demonstrated. Compared to pure CMM or carbon nanotubes, CMM containing Pt-NPs enhances the electron transfer and redox properties of redox enzymes, such as glucose oxidase (GOx), due to a cooperative effect of Pt-NPs and CMM. The quasi-reversible electron transfer of GOx in this system is probed, and the apparent heterogeneous electron transfer rate constants are found to be 66% larger than in pure CMM. The GOx/Pt-CMM based glucose biosensor enables the determination of glucose at a potential of 600 mV (vs. SCE). Its detection limit is 10 times lower, and the sensitivity is 16 times higher than that of the respective biosensor without Pt-NPs.
引用
收藏
页码：109 / 116
页数：8
相关论文
共 50 条
  • [1] Electrochemistry and biosensing of glucose oxidase immobilized on Pt-dispersed mesoporous carbon
    Chunping You
    Xiang Li
    Song Zhang
    Jilie Kong
    Dongyuan Zhao
    Baohong Liu
    Microchimica Acta, 2009, 167
  • [2] Direct electrochemistry and biocatalysis of glucose oxidase immobilized on magnetic mesoporous carbon
    Jingjing Yu
    Jiaxing Tu
    Faqiong Zhao
    Baizhao Zeng
    Journal of Solid State Electrochemistry, 2010, 14 : 1595 - 1600
  • [3] Direct electrochemistry and biocatalysis of glucose oxidase immobilized on magnetic mesoporous carbon
    Yu, Jingjing
    Tu, Jiaxing
    Zhao, Faqiong
    Zeng, Baizhao
    JOURNAL OF SOLID STATE ELECTROCHEMISTRY, 2010, 14 (09) : 1595 - 1600
  • [4] Direct electrochemistry of glucose oxidase immobilized on mesoporous TiO2
    Wu, Xinbing
    Meng, Meng
    Zhuang, Wei
    Lü, Linghong
    Lu, Xiaohua
    Huagong Xuebao/CIESC Journal, 2014, 65 (05): : 1777 - 1783
  • [5] Direct electrochemistry and reagentless biosensing of glucose oxidase immobilized on chitosan wrapped single-walled carbon nanotubes
    Zhou, Yi
    Yang, Hui
    Chen, Hong-Yuan
    TALANTA, 2008, 76 (02) : 419 - 423
  • [6] Electrochemistry of glucose oxidase immobilized on the carbon nanotube wrapped by polyelectrolyte
    Wen, Dan
    Liu, Ying
    Yang, Guocheng
    Dong, Shaojun
    ELECTROCHIMICA ACTA, 2007, 52 (16) : 5312 - 5317
  • [7] Direct electrochemistry of glucose oxidase immobilized on surface of activated carbon
    Sun, DM
    Cai, CX
    Xing, W
    Lu, TH
    CHINESE JOURNAL OF INORGANIC CHEMISTRY, 2005, 21 (03) : 405 - 408
  • [8] Electrochemistry and biosensing of glucose oxidase based on mesoporous carbons with different spatially ordered dimensions
    You, Chunping
    Xu, Xin
    Tian, Bozhi
    Kong, Jilie
    Zhao, Dongyuan
    Liu, Baohong
    TALANTA, 2009, 78 (03) : 705 - 710
  • [9] Direct Electrochemistry of Glucose Oxidase on Nail-like Carbon and Its Biosensing for Glucose
    Li, Kuangtian
    Liu, Bin
    Zheng, Jianbin
    Sheng, Qinglin
    Liu, Ruixiao
    ELECTROANALYSIS, 2010, 22 (06) : 701 - 706
  • [10] Direct electrochemistry of glucose oxidase immobilized on carbon nanotube for improving glucose sensing
    Hyun, Kyuhwan
    Han, Sang Won
    Koh, Won-Gun
    Kwon, Yongchai
    INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2015, 40 (05) : 2199 - 2206
12345