Molecular characterisation of Victorian Newcastle disease virus isolates from 1976 to 1999

被引：6
|
作者：
Peroulis-Kourtis, I [1 ]
O'Riley, K [1 ]
Grix, D [1 ]
Condron, RJ [1 ]
Ainsworth, C [1 ]
机构：
[1] Victorian Inst Anim Sci, Dept Nat Resources & Environm, Attwood, Vic 3049, Australia
来源：
AUSTRALIAN VETERINARY JOURNAL | 2002年 / 80卷 / 07期
关键词：
D O I：
10.1111/j.1751-0813.2002.tb11004.x
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Objective To characterise Newcastle disease virus isolates obtained in Victoria from 1976 to 1999 and identify the diversity of F0 cleavage signal. Design RT-PCR using viral RNA extracted from positive NDV allantoic fluid was performed to amplify a segment of the NDV F and HN genes. Molecular characterisation of the nucleotide and amino acid sequences within the F0 cleavage site was undertaken. Results All isolates contained 'avirulent' F0 cleavage signal sequence of varied amino acid composition. Conclusions Molecular characterisation of past and present NDV F0 cleavage signal sequences will provide valuable epidemiological information and assist in understanding the genetic origins and relationships of outbreaks.
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收藏
页码：422 / 424
页数：3
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