Restriction of the CD4+ T-cell receptor repertoire prevents immune pathology in TGF-β1 knockout mice

Mice with a targeted deletion in TGF-beta 1 spontaneously develop CD4(+) T-cell-dependent multifocal inflammatory disease and autoimmune pathology. T cells from TGF-beta 1(-/-) mice are strongly activated, but the mechanisms that lead to T-cell activation and organ pathology are not well understood. Recent work shows that TGF-beta 1 raises the threshold for signaling through the TCR, suppressing the response of T cells to mitogenic stimuli. This suggests the possibility that CD4(+) T cells in TGF-beta 1(-/-) mice become aberrantly activated and cause damage in response to physiologic inputs that ordinarily are not sufficient for cell activation, such as homeostatic MHC-TCR interactions, cytokines, or adhesion molecules. This model predicts that pathology is largely antigen-independent, and that CD4(+) T cells, regardless of antigen specificity, will become activated in TGF-beta 1(-/-) mice, with subsequent organ pathology. To test this model, we crossed BALB/c-TGF-beta 1(-/-) mice with the DO11.10 TCR transgenic mouse. To obviate the possible development of nonclonotypic TCRs, we also bred in a deficiency in RAG-1. Cohorts of highly inbred BALB/c background TGF-beta 1(-/-) mice with an increasingly restricted CD4(+) T-cell repertoire ( TGF-beta 1(-/-) mice; DO11.10-TGF-beta 1(-/-) mice; DO11.10-RAG-1(-/-) TGF-beta 1(-/-) mice) were then analyzed for inflammatory organ pathology and T-cell activation. The data show that progressively restricting the CD4(+) T-cell repertoire improved survival, ameliorated target organ pathology, and reduced T-cell activation to control levels. Therefore, these results find no support for the involvement of atypical T-cell activation pathways in disease in TGF-beta 1(-/-) mice. Rather, T-cell activation and pathology in TGF-beta 1(-/-) mice appear to be functions of typical TCR activation pathways. This supports the hypothesis that immune pathology in TGF-beta 1(-/-) mice is self-antigen triggered.