Dietary vitamin D ameliorates hepatic oxidative stress and inflammatory effects of diethylnitrosamine in rats

被引:9
|
作者
Adelani, I. B. [1 ]
Ogadi, E. O. [1 ]
Onuzulu, C. [1 ]
Rotimi, O. A. [1 ]
Maduagwu, E. N. [2 ]
Rotimi, S. O. [1 ]
机构
[1] Covenant Univ, Coll Sci & Technol, Dept Biochem, Ota, Ogun State, Nigeria
[2] Chrisland Univ, Dept Biochem, Abeokuta, Ogun State, Nigeria
关键词
Nutrition; Biochemistry; Toxicology; Diet; Vitamin D; Antioxidant; Oxidative stress; Inflammation; Anti-inflammation; Diethylnitrosamine; GLUTATHIONE-S-TRANSFERASE; D DEFICIENCY; HEPATOCELLULAR-CARCINOMA; CANCER; PROGRESSION; MECHANISMS; RELEVANCE; INJURY; RISK;
D O I
10.1016/j.heliyon.2020.e04842
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The generation of reactive oxygen species (ROS) plays an essential role in the pathogenesis of several diseases. Its implication in inflammation has suggested a possible link between oxidative stress and activation/release of cytokines in precancerous states. Recent observational studies have suggested an association between inflammation and vitamin D deficiency; hence, suggesting that vitamin D could play a role in the pathogenesis of diseases. This study examined the antioxidant and anti-inflammatory potentials of vitamin D in diethylnitrosamine (DEN)-induced oxidative stress and inflammation in rats. Rats were divided into four experimental groups. While groups one and two were administered twice weekly with 30 mg/kg body weight DEN for six weeks, groups three and four were given normal saline. Groups one and three were fed with vitamin D deficient diet, while groups two and four were fed vitamin D diet during the experiment. After that, biomarkers of oxidative stress status were assayed spectrophotometrically. The concentration of inflammatory cytokines was determined using enzyme-linked immunosorbent assay (ELISA). DEN-induced vitamin D deficient diet group had increased antioxidant enzymes' activities. Also, there were elevated concentrations of thiobarbituric acid reactive substances (TBARS) and inflammatory cytokines in the same group. Vitamin D diet, however, reduced oxidative stress effects through the reduction in the activities of TBARS and caused a significant (p < 0.05) increase in nitric oxide concentration. Vitamin D diet significantly (p < 0.05) reduced the level of interleukin 1 beta and TNF-alpha produced in the deficiency state. These findings show that vitamin D may play an essential role in the regulation of hepatic oxidative stress and inflammatory responses.
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页数:8
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