Biochemical Characterisation of α-Amylase in Two Aphid Species, Aphis fabae Scopoli (Hemiptera: Aphididae) and A. gossypii Glover (Hemiptera: Aphididae)

被引：5
|
作者：
Darvishzadeh, Ali [1 ]
Bandani, Ali Reza [1 ]
Mousavi, Seyed Qasem [1 ]
机构：
[1] Univ Tehran, Dept Plant Protect, Coll Agr & Nat Resources, Karaj, Alborz, Iran
来源：
PLANT PROTECTION SCIENCE | 2014年 / 50卷 / 02期
关键词：
aphids; gel assay; starch; PROTEIN DIGESTION; MYZUS-PERSICAE; MIDGUT; INSECT; IDENTIFICATION; PURIFICATION; RESISTANCE; INHIBITORS; LINNAEUS; CLONING;
D O I：
10.17221/71/2012-PPS
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
We identify and characterise alpha-amylases of the two aphid species, A. fabae and A. gossypi. To do this, alpha-amylases of the two insect species were extracted and their activities were determined using 1% soluble starch. Results showed that alpha-amylase, which hydrolyses starch, is present in both aphids. Also, it was shown that optimum pH and temperature for the alpha-amylases of both species is 7.0 and 40 degrees C, respectively. Gel assays using zymogram analysis showed that in both aphid species more than one isoform (two isoforms) of alpha-amylases hydrolyszing carbohydrates are present.
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页码：84 / 89
页数：6
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