Sex-specific markers undetected in green sunfish Lepomis cyanellus using restriction-site associated DNA sequencing

被引：2
|
作者：
Teal, Chad N. [1 ]
Coykendall, D. Katharine [2 ,3 ]
Campbell, Matthew R. [3 ]
Eardley, Daniel L. [2 ,3 ]
Delomas, Thomas A. [2 ,3 ]
Shira, James T. [4 ]
Schill, Daniel J. [5 ]
Bonar, Scott A. [6 ]
Culver, Melanie [6 ]
机构：
[1] Sch Nat Resources & Environm, Arizona Cooperat Fish & Wildlife Res Unit, Tucson, AZ USA
[2] Pacific States Marine Fisheries Commiss, Eagle Fish Genet Lab, Eagle, ID USA
[3] Idaho Dept Fish & Game, Eagle Fish Genet Lab, Eagle, ID USA
[4] Univ Arizona Genet Core, Tucson, AZ USA
[5] Fisheries Management Solut Inc, Boise, ID USA
[6] Univ Arizona, US Geol Survey, Sch Nat Resources & Environm, Arizona Cooperat Fish & Wildlife Res Unit, ENR2, Tucson, AZ 85721 USA
来源：
JOURNAL OF FISH BIOLOGY | 2022年 / 100卷 / 06期
关键词：
centrarchid; markers; RAD-seq; sex determination; sex identification; TROJAN Y-CHROMOSOME; RAD-SEQ; IDENTIFICATION; POPULATION; RATIO; FISH; TRANSITIONS; SUPPRESSION; ERADICATION; MACROCHIRUS;
D O I：
10.1111/jfb.15063
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
We used restriction-site associated DNA sequencing for SNP discovery and genotyping of known-sex green sunfish Lepomis cyanellus DNA samples to search for sex-diagnostic single nucleotide polymorphisms (SNPs) and restriction-site associated sequences present in one sex and absent in the other. The bioinformatic analyses discovered candidate SNPs and sex-specific restriction-site associated sequences that fit patterns of male or female heterogametic sex determination systems. However, when primers were developed and tested, no candidates reliably identified phenotypic sex. The top performing SNP candidate (ZW_218) correlated with phenotypic sex 63.0% of the time and the presence-absence loci universally amplified in both sexes. We recommend further investigations that interrogate a larger fraction of the L. cyanellus genome. Additionally, studies on the effect of temperature and rearing density on sex determination, as well as breeding of sex-reversed individuals, could provide more insights into the sex determination system of L. cyanellus.
引用
收藏
页码：1528 / 1540
页数：13
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