Molecular Cloning and Expression Patterns Study of Growth Differentiation Factor 9 Gene (GDF9) in Water Buffalo (Bubalus bubalis)

被引:1
|
作者
Liu, Jinfeng [1 ,2 ]
Cao, Yanhong [3 ]
Wu, Zhuyue [3 ]
Shafique, Laiba [1 ]
Chen, Shaomei [3 ]
Xuan, Zeyi [3 ]
Peng, Tong [1 ]
Luo, Chan [1 ]
Zhu, Peng [1 ,4 ]
Liu, Qingyou [1 ]
机构
[1] Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530005, Guangxi, Peoples R China
[2] Guangxi Vet Res Inst, Guangxi Key Lab Vet Biotechnol Nanning, Nanning 530001, Guangxi, Peoples R China
[3] Anim Husb Res Inst Guangxi Zhuang Autonomous Reg, Nanning 530001, Peoples R China
[4] Qinzhou Univ, Guangxi Key Lab Beibu Gulf Marine Biodivers Conse, Qinzhou 530005, Guangxi, Peoples R China
关键词
Buffalo; GDF9; Expression pattern; Oogenesis; BONE MORPHOGENETIC PROTEIN-15; IN-VITRO MATURATION; FOLLICLE DEVELOPMENT; IDENTIFICATION; OOCYTES; BMP15;
D O I
10.17957/IJAB/15.1162
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The Gene Growth Differentiation Factor 9 (GDF9) is far most important gene for oogenesis. Few studies have been reported concerning buffalo oogenesis and embryogenesis. In current study, complete coding region of a gene of the buffalo GDF9 gene was cloned and sequenced, which was found to have 1,362 nucleotides long encoding of 453 amino acid proteins. The expression pattern analysis results showed that GDF9 existed spanning the entire stage of folliculogenesis and embryogenesis. The GDF9 gene increased in the initial stage then decreased during follicular development. The gene was significantly higher in the Cumulus-Oocyte-Complexes (COC) of small and middle diameter follicles compared to large follicles. Its expression level was significantly higher in embryogenesis rather than folliculogenesis except the blastula stage Immunohistochemistiy showed that the GDF9 protein was found in testis and in follicles of oocytes including primordial granulose cells, primary, secondary and antral follicles, and also in theca cells. The study of the GDF9 gene provided an important base knowledge for further study regulation mechanism of GDF9 gene in buffalo oogenesis. (C) 2019 Friends Science Publishers
引用
收藏
页码:1010 / 1016
页数:7
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