Endogenous RGS proteins facilitate dopamine D2S receptor coupling to Gαo proteins and Ca2+ responses in CHO-K1 cells

被引：11
|
作者：
Boutet-Robinet, EA [1 ]
Finana, F [1 ]
Wurch, T [1 ]
Pauwels, PJ [1 ]
De Vries, L [1 ]
机构：
[1] Ctr Rech Pierre Fabre, Dept Cell & Mol Biol, F-81106 Castres, France
来源：
FEBS LETTERS | 2003年 / 533卷 / 1-3期
关键词：
RGS; dopamine D-2 receptor; G protein coupling; GTP gamma S; Ca2+ response;
D O I：
10.1016/S0014-5793(02)03753-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The role of RGS proteins on dopaminergic Des receptor (D2SR) signalling was investigated in Chinese hamster ovary (CHO)-K1 cells, using recombinant RGS protein- and PTX-insensitive G(alpha0) proteins. Dopamine-mediated [S-35]GTPgammaS binding was attenuated by more than 60% in CHO-K1 D2SR cells coexpressing a RGS protein- and PTX-insensitive G(alpha0)Gly(184)Ser:Cys(351)Ile protein versus cells coexpressing a similar amount of PTX-insensitive G(alpha0)Cys(351)Ile protein. Dopamine-agonist-mediated Ca2+ responses were dependent on the coexpression with a G(alpha0)Cys(351)Ile protein and were fully abolished upon coexpression with a G(alpha0)Gly(184)Ser:Cys(351)Ile protein. These results suggest that interactions between the G(alpha0) protein and RGS proteins are involved in efficient D2SR signalling. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：67 / 71
页数：5
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