RAPD analysis for genetic diversity of two populations of Mystus vittatus (Bloch) of Madhya Pradesh, India

被引：0
|
作者：
Garg, R. K. [1 ]
Silawat, N. [1 ]
Sairkar, Pramod [1 ]
Vijay, Neetu [1 ]
Mehrotra, N. N. [2 ]
机构：
[1] MPCST Vigyan Bhawan, MP Council Sci & Technol, Ctr Excellence Biotechnol, Bhopal 462003, MP, India
[2] Cent Drug Res Inst, Lucknow 226001, Uttar Pradesh, India
来源：
AFRICAN JOURNAL OF BIOTECHNOLOGY | 2009年 / 8卷 / 17期
关键词：
Catfish; genetic diversity; RAPD; polymorphism; AMPLIFIED POLYMORPHIC DNA; MITOCHONDRIAL-DNA; TENUALOSA-ILISHA; IDENTIFICATION; HAMILTON; MARKERS; FISHES; SHAD;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation of the 2 populations of Mystus vittatus (Bloch) of Madhya Pradesh, India. 10 random 10-mer primers were primarily scored in 3 individuals from each of the 2 locations. Five primers, which gave polymorphism, were selected for polymerase chain reaction (PCR) and used in the final RAPD analysis of 20 individuals collected from 2 sites, namely Bhadwada reservoir (Bhopal) and Mohinisagar reservoir (Gwalior). These primers produced 388 scorable DNA fragments were found, of which 252 (64.98%) were polymorphic, 182 (46.90%) were monomorphic and 14 (3.61%) were unique. RAPD banding patterns, showed variations between and within the populations, while, the morphological variations were negligible.
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页码：4032 / 4038
页数：7
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