Genetic transformation of wheat (Triticum aestivum L) by Agrobacterium tumefaciens

Genetic transformation. of spring, common wheat (Triticum aestivum L.) using Agrobacterium tumefaciens was performed. Agrobacterium strain C158, which contains the cointegrate of plasmids pCV2260 and pSIR42, was used. Agrobacterium cells were applied to flower pistils after artificial pollination. The seed set was 3%. F-1 plants were morphologically similar to the control. Genomic DNA analysis by dot hybridization and PCR confirmed the existence of recombinant DNA in some plants. F-2 was obtained from the transformed plants; these plants also contained foreign DNA. Repeated transformation by this method gave the same result. Transformation frequency was at least 2.7%.