Monoclonal antibody E8-18 identifies an integral membrane surface protein unique to Mycoplasma capricolum subsp. capripneumoniae

被引：2

作者：

Rurangirwa, FR ^{[1
]}

Shompole, PS ^{[1
]}

Wambugu, AN ^{[1
]}

Kihara, SM ^{[1
]}

McGuire, TC ^{[1
]}

机构：

[1] KENYA AGR RES INST,NATL VET LAB,BIOTECHNOL & IMMUNOL SECT,KABETE,KENYA

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 1997年 / 4卷 / 05期

关键词：

D O I：

10.1128/CDLI.4.5.615-619.1997

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Monoclonal antibody (MAb) E8-18 reacted with four isolates of Mycoplasma capricolum subsp. capripneumoniae in Western blots identifying an epitope on a 24 kDa antigen (p24). MAb E8-18 did not react with II isolates belonging to four other Mycoplasma species or subspecies closely related to M. capricolum subsp. capripneumoniae. A combination of trypsin treatment of intact organisms and detergent-phase partitioning revealed p24 to be an integral M. capricolum subsp. capripneumoniae surface membrane protein.

引用

页码：615 / 619

页数：5

共 5 条

[1] Antibody response to Mycoplasma capricolum subsp. capripneumoniae bacterium in small holder dairy goats in Tanzania
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[2] Phage displayed peptides and anti-idiotype antibodies recognised by a monoclonal antibody directed against a diagnostic antigen of Mycoplasma capricolum subsp capripneumoniae
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[3] Development and Evaluation of an ImmunoCapture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. Capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine (vol 2020, pg 10, 2020)
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[4] TRITON X-114 PHASE FRACTIONATION OF AN INTEGRAL MEMBRANE-SURFACE PROTEIN MEDIATING MONOCLONAL-ANTIBODY KILLING OF MYCOPLASMA-HYORHINIS
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[5] MONOCLONAL-ANTIBODY HFD9 IDENTIFIES A NOVEL 28 KDA INTEGRAL MEMBRANE-PROTEIN ON THE CIS-GOLGI
SUBRAMANIAM, VN
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