Evaluation of the antifungal activity in vitro of midazolam against fluconazole-resistant Candida spp. isolates

被引：10
|
作者：
Holanda, Maria A., V [1 ,2 ]
da Silva, Cecilia R. [1 ,2 ]
Neto, Joao B. de A. [1 ,2 ,3 ]
Sa, Livia G. do A., V [1 ,2 ]
do Nascimento, Francisca B. S. A. [1 ,2 ]
Barroso, Daiana D. [1 ,2 ]
da Silva, Lisandra J. [1 ,2 ]
Candido, Thiago M. [1 ,2 ,3 ]
Leitao, Amanda C. [1 ,2 ]
Barbosa, Amanda D. [1 ,2 ]
de Moraes, Manoel O. [2 ]
Moraes, Bruno C. C. [2 ]
Moraes, Helio V. N. J. [1 ,2 ]
机构：
[1] Univ Fed Ceara, Lab Bioprospect Antimicrobial Mol LABIMAN, Sch Pharm, Dept Clin & Toxicol Anal, BR-60430116 Fortaleza, Ceara, Brazil
[2] Univ Fed Ceara, Drug Res & Dev Ctr, BR-60430276 Fortaleza, Ceara, Brazil
[3] Univ Ctr Christus, BR-60160230 Fortaleza, Ceara, Brazil
来源：
FUTURE MICROBIOLOGY | 2021年 / 16卷 / 02期
关键词：
antifungal potential; biofilm; Candida spp; drug repositioning; midazolam; OXYGEN SPECIES ROS; TROPICALIS RESISTANT; ANTICANDIDA ACTIVITY; CELL-DEATH; APOPTOSIS; GROWTH; ALBICANS; WALL;
D O I：
10.2217/fmb-2020-0080
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: The purpose of this study was to evaluate the antifungal activity of midazolam, alone and in association with azoles, against isolates of clinical Candida spp. in planktonic and biofilm form. Materials & methods: The antifungal activity was observed using the broth microdilution technique. Flow cytometry tests were performed to investigate the probable mechanism of action and the comet test and cytotoxicity test were applied to evaluate DNA damage. Results: Midazolam (MIDAZ) showed antifungal activity against planktonic cells (125-250 mu g/ml) and reduced the viability of Candida spp. biofilms (125 a 2500 mu g/ml). The interaction of MIDAZ against Candida spp. biofilms was observed through scanning electron microscopy, causing alteration of their appearance. Therefore, MIDAZ has antifungal potential against Candida spp.
引用
收藏
页码：71 / 81
页数：11
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