T-cell responses to the Mycobacterium tuberculosis- specific antigen ESAT-6 in Brazilian tuberculosis patients

被引:59
|
作者
Cardoso, FLL
Antas, PRZ
Milagres, AS
Geluk, A
Franken, KLMC
Oliveira, EB
Teixeira, HC
Nogueira, SA
Sarno, EN
Klatser, P
Ottenhoff, THM
Sampaio, EP
机构
[1] Fiocruz MS, Leprosy Lab, Inst Oswaldo Cruz, BR-21045900 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Dept Infect & Parasit Dis, Hosp Univ Clementino Frago Filho, BR-21941 Rio De Janeiro, Brazil
[3] Hosp Municipal Raphael Paula & Souza, Dept Lung Dis, Rio De Janeiro, Brazil
[4] Univ Fed Juiz de Fora, Immunol Lab, Juiz De Fora, Brazil
[5] Leiden Univ, Med Ctr, Dept Immunohematol & Blood Transfus, NL-2300 RA Leiden, Netherlands
[6] Royal Trop Inst, Dept Biomed Res, NL-1105 AZ Amsterdam, Netherlands
关键词
D O I
10.1128/IAI.70.12.6707-6714.2002
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The Mycobacterium tuberculosis-specific ESAT-6 antigen induces highly potent T-cell responses and production of gamma interferon (IFN-gamma), which play a critical role in protective cell-mediated immunity against tuberculosis (TB). In the present study, IFN-gamma secretion by peripheral blood mononuclear cells (PBMCs) in response to M. tuberculosis ESAT-6 in Brazilian TB patients was investigated in relation to clinical disease types, such as pleurisy and cavitary pulmonary TB. Leprosy patients, patients with pulmonary diseases other than TB, and healthy donors were assayed as control groups. Sixty percent of the TB patients indeed recognized M. tuberculosis ESAT-6, as did 50% of the leprosy patients and 60% of the non-TB controls. Nevertheless, the levels of IFN-gamma in response to the antigen ESAT, but not to antigen 85B (Ag85B) and purified protein derivative (PPD), were significantly lower in controls than in patients with treated TB or pleural or cavitary TB. Moreover, according to Mycobacterium bovis BCG vaccination status, only 59% of the vaccinated TB patients responded to ESAT in vitro, whereas 100% of them responded to PPD. Both CD4 and CD8 T cells were able to release IFN-gamma in response to ESAT. The present data demonstrate the specificity of ESAT-6 of M. tuberculosis and its ability to discriminate TB patients from controls, including leprosy patients. However, to obtain specificity, it is necessary to include quantitative IFN-gamma production in response to the antigen as well, and this might limit the use of ESAT-6-based immunodiagnosis of M. tuberculosis infection in an area of TB endemicity.
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页码:6707 / 6714
页数:8
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