Molecular characterization and pathogenicity of tomato yellow leaf curl virus in China

被引：108
|
作者：
Zhang, Hui [1 ,2 ]
Gong, Huanran [1 ]
Zhou, Xueping [1 ]
机构：
[1] Zhejiang Univ, Inst Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects MOA, Hangzhou 310029, Zhejiang, Peoples R China
[2] Shanghai Acad Agr Sci, Inst Hort Res, Shanghai 201106, Peoples R China
来源：
VIRUS GENES | 2009年 / 39卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Tomato yellow leaf curl virus; Sequence; Infectivity; China; WHITEFLY-TRANSMITTED GEMINIVIRUSES; DNA-BETA; MONOPARTITE BEGOMOVIRUS; GENOME ORGANIZATION; NUCLEOTIDE-SEQUENCE; YUNNAN-VIRUS; DISEASE; TOBACCO; DEMARCATION; CROP;
D O I：
10.1007/s11262-009-0384-8
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Several tomato production regions in China were surveyed for tomato yellow leaf curl disease (TYLCD), and 31 tomato leaf samples showing TYLCD-like symptoms were collected. The partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (TYLCV) was detected in Shanghai, Zhejiang, Jiangsu Shandong and Hebei provinces of China. The TYLCV isolates found in China share high sequence identity (> 98%) and have more than 97% sequence identity with TYLCV-IL[IL:Reo] (X15656). Phylogenetic relationship analysis reveals that although with little genetic variability, they can form two groups and all the TYLCV isolates in China belong to the group I. An infectious clone of TYLCV-[CN:SH2] (AM282874) was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum, Petunia hybrida, Cucumis sativus, Gossypium hirsutum, S. melongena, and Capsicum annuum. TYLCV-[CN:SH2] can induce severe leaf curling and stunting symptoms in these plants except C. sativus, G. hirsutum, S. melongena and C. annuum. We verified that TYLCV can trans-replicate tomato yellow leaf curl China virus DNA-beta in N. benthamiana and S. lycopersicum and induced more severe symptoms with distortion and yellow vein.
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收藏
页码：249 / 255
页数：7
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