Direct electron transfer between hemoglobin and a glassy carbon electrode facilitated by lipid-protected gold nanoparticles

被引：85

作者：

Han, XJ ^{[1
]}

Cheng, WL ^{[1
]}

Zhang, ZL ^{[1
]}

Dong, SJ ^{[1
]}

Wang, EK ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2002年 / 1556卷 / 2-3期

基金：

中国国家自然科学基金;

关键词：

hemoglobin; lipid-protected nanoparticle; direct electron transfer; UV-Vis spectrum;

D O I：

10.1016/S0005-2728(02)00372-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We synthesized a kind of gold nanoparticle protected by a synthetic lipid (didodecyidimethylammonium bromide, DDAB). With the help of these gold nanoparticles, hemoglobin can exhibit a direct electron transfer (DET) reaction. The formal potential locates at -169 mV vs. Ag/AgCl. Spectral data indicated the hemoglobin on the electrode was not denatured. The lipid-protected gold nanoparticles were very stable (for at least 8 months). Their average diameter is 6.42 nm. It is the first time to use monolayer-protected nanoparticles to realize the direct electrochemistry of protein. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：273 / 277

页数：5

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