Fluorescence quenching detection of peanut agglutinin based on photoluminescent silole-core carbosilane dendrimer peripherally functionalized with lactose

被引：24

作者：

Hatano, Ken ^{[1
]}

Saeki, Hitoshi ^{[1
]}

Yokota, Hiroo ^{[1
]}

Aizawa, Hiroaki ^{[1
]}

Koyama, Tetsuo ^{[1
]}

Matsuoka, Koji ^{[1
]}

Terunuma, Daiyo ^{[1
]}

机构：

[1] Saitama Univ, Grad Sch Sci & Technol, Div Mat Sci, Sakura Ku, Saitama 3388570, Japan

来源：

TETRAHEDRON LETTERS | 2009年 / 50卷 / 42期

基金：

日本学术振兴会;

关键词：

Aggregation-induced emission; Biosensor; Carbohydrate; Fluorescence quenching; Glycocluster; Lectin; Silole; BINDING; LECTIN; SACCHARIDES; DESIGN;

D O I：

10.1016/j.tetlet.2009.07.153

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A glycocluster peripherally functionalized with a lactose (Lac: Gal beta 1 -> 4Glc beta 1-) derivative possessing a silole moiety as a luminophore was synthesized. The photoluminescence spectrum of the glycocluster showed extremely strong emission at 474 nm and the absolute quantum yield was estimated to be 92% in distilled water. The emission intensity was decreased by increasing the amount of peanut agglutinin (PNA), a lactose-binding lectin, and plots of the relative fluorescence intensity revealed a decline of 95% in emission intensity. Fluorescence quenching of the glycocluster upon mixing with PNA could be easily observed by the naked eye under UV irradiation, whereas no distinct change in fluorescence properties of the glycocluster was observed when wheat germ agglutinin (WGA) was employed. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：5816 / 5819

页数：4