Presence of a Na+/H+ exchanger in acidocalcisomes of Leishmania donovani and their alkalization by anti-leishmanial drugs

被引：50
|
作者：
Vercesi, AE
Rodrigues, CO
Catisti, R
Docampo, R
机构：
[1] Univ Illinois, Dept Pathobiol, Mol Parasitol Lab, Urbana, IL 61802 USA
[2] Univ Estadual Campinas, Fac Ciencias Med, Dept Patol Clin, BR-13083970 Campinas, SP, Brazil
来源：
FEBS LETTERS | 2000年 / 473卷 / 02期
关键词：
acidocalcisome; Na+/H+ exchanger; 3,5-dibutyl-4-hydroxytoluene; volutin; Leishmania donovani;
D O I：
10.1016/S0014-5793(00)01531-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Acidocalcisomes are acidic vacuoles present in trypanosomatids that contain most of the cellular calcium. The data presented here demonstrate that Leishmania donovani acidocalcisomes possess a Na+/H+ exchanger. 3,5-Dibutyl-4-hydroxytoulene, in the concentration range of 0-20 mu M, inhibited the Na+/H+ exchanger, and strongly stimulated the activity of the vacuolar H+-ATPase responsible for vacuolar acidification. As occurs with Na+, the cationic anti-leishmanial drugs pentamidine, WR-6026, and chloroquine promoted a fast and extensive alkalization of the L, donovani acidocalcisomes. (C) 2000 Federation of European Biochemical Societies.
引用
收藏
页码：203 / 206
页数：4
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