Effects of elevated temperature, elevated CO2 and fertilization on quality and subsequent decomposition of silver birch leaf litter

We examined the quality and decomposition of naturally abscised leaves of silver birch (Betula pendula) seedlings subjected to three different levels of fertilization under ambient and elevated levels of temperature and CO2. At the end of the second growing season, the chemical composition of the litter collected from the seedlings was analyzed. Whole-leaf samples from pooled litter from each of the four replicates from each treatment were put in mesh bags and transferred to ambient climate in the field. The remaining mass of litter was measured by sampling bags in May and October throughout the four-year incubation period. Fertilization with all nutrients decreased the initial carbon and tannin contents of litter, and increased the proportion of the fast-decomposing fraction, but still fertilization slowed down the decomposition of this fraction. Initially, the estimated proportion of the fast-decomposing fraction was smallest in elevated CO2 + temperature, and largest in ambient climate. During decomposition, elevated growth-temperature slowed down decomposition of the fast fraction under ambient CO2 but increased it under elevated CO2. The changes in litter decomposition rates found over four years were not very large. However, we conclude that the interactions of different factors lead to different results than if the factors had been studied separately, and future studies should take interactions into account. (C) 2009 Elsevier Ltd. All rights reserved.