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Detection of trisomy 12 by fluorescence in situ hybridization on archival cytopathologic material in chronic lymphocytic leukemia/small lymphocytic lymphoma
被引:1
|作者:
Liu, K
[1
]
Dodd, LG
[1
]
Powers, JA
[1
]
Bigner, SH
[1
]
机构:
[1] Duke Univ, Med Ctr, Dept Pathol, Div Cytopathol, Durham, NC 27710 USA
关键词:
trisomy;
in situ hybridization;
fluorescence;
leukemia;
lymphocytic;
chronic;
aspiration biopsy;
small lymphocytic lymphoma;
12;
D O I:
10.1159/000328480
中图分类号:
R36 [病理学];
学科分类号:
100104 ;
摘要:
OBJECTIVE: To evaluate fluorescence in situ hybridization (FISH) for the detection of trisomy 12 in archival cytologic specimens of chronic lymphocytic leukemia/ small lymphocytic lymphoma. STUDY DESIGN: The cytopathology database was searched for all the cases of chronic lymphocytic lymphoma. Six cases of chronic lymphocytic leukemia/small lymphocytic lymphoma obtained by fine needle aspiration and one case of small lymphocytic lymphoma with plasmacytoid features were analyzed for trisomy 12 by FISH. These cases had been archived between 1 week to 16 months prior to analysis. RESULTS: We decided trisomy 12 in four of the six cases of small lymphocytic lymphoma/chronic lymphocytic leukemia. The case of small lymphocytic lymphoma with plasmacytoid features was negative for trisomy 12. CONCLUSION: Detection of trisomy 12 by FISH can be effectively performed on routinely prepared, stained and coverslipped archival cytologic material.
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页码:368 / 374
页数:7
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