Quasi-collimated beam apparatus (QCBA), a typical bench scale UV apparatus, is crucial for the biodosimetry determination of UV dose in target reactors. However, the key parameters for the QCBA construction are usually estimated via rule-of-thumb calculations. Computational fluid dynamics models are applied in this study to simulate the UV fluence rate (FR) distributions in QCBAs. QCBAs with either a cylindrical tube or successive apertures irradiate quasi parallel light into selected dishes. The simulated Petri factor (PF) in the target QCBAs with a single aperture were all >0.84, and increased with the extended distance (L-1) from the UV lamp to the upper aperture. QCBAs with two successive apertures are recommended compared with those with three apertures or cylindrical tube. A trend of FR distribution from dispersed to concentrated is observed when L-1 or the interval distance between each aperture increases in a dual-aperture QCBA. QCBAs with multiple lamps were favorable to increase the UV output power, while having a nearly negligible loss of parallelism. An actual QCBA was constructed, and the maximal and average FR, and PF values in a 60-mm dish were 0.159 and 0.164 W/m(2), and 0.967, respectively, in accordance with the simulated results.