Cloning and identification of magnetic field-responsive genes in Daudi cells

被引：1
|
作者：
Wu, RY [1 ]
Jiang, HA [1 ]
Hu, GL [1 ]
Fu, YT [1 ]
Lu, DQ [1 ]
机构：
[1] Zhejiang Univ, Sch Med, Microwave Inst, Hangzhou 310006, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2000年 / 45卷 / 11期
基金：
中国国家自然科学基金;
关键词：
ELF magnetic field; mRNA differential display; responsive genes;
D O I：
10.1007/BF02884981
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In order to study the molecular mechanisms of ELF MF biological effects, the differential display (DD) technology has been used to isolate differentially expressed cDNA fragments in Daudi cells after 50 Hz MF exposure and sham-exposure. Using total RNA isolated from 0.8 mT 50 Hz MF exposed cells and those from sham-exposed cells, one differentially regulated transcript has been found by DD and the expression pattern of the same transcript has been verified by reverse-Northern and Northern analyses. DNA sequence analysis and Genbank research demonstrated that the cDNA fragment (MF-CA) is a novel gene in human cell which is induced by ELF MF.
引用
收藏
页码：1006 / 1010
页数：5
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