Human Platelet Lysate as a Functional Substitute for Fetal Bovine Serum in the Culture of Human Adipose Derived Stromal/Stem Cells
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作者:
Cowper, Mathew
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Tulane Univ, Sch Med, New Orleans, LA 70112 USA
Wake Forest Univ, Bowman Gray Sch Med, Dept Urol, Winston Salem, NC 27101 USATulane Univ, Sch Med, New Orleans, LA 70112 USA
Cowper, Mathew
[1
,7
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Frazier, Trivia
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机构:
Tulane Univ, Sch Med, New Orleans, LA 70112 USA
LaCell LLC, New Orleans, LA 70148 USA
Obatala Sci Inc, New Orleans, LA 70148 USATulane Univ, Sch Med, New Orleans, LA 70112 USA
Frazier, Trivia
[1
,2
,3
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Wu, Xiying
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机构:
LaCell LLC, New Orleans, LA 70148 USA
Obatala Sci Inc, New Orleans, LA 70148 USATulane Univ, Sch Med, New Orleans, LA 70112 USA
Wu, Xiying
[2
,3
]
Curley, J. Lowry
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机构:
LaCell LLC, New Orleans, LA 70148 USA
Axosim Inc, New Orleans, LA 70112 USATulane Univ, Sch Med, New Orleans, LA 70112 USA
Curley, J. Lowry
[2
,4
]
Ma, Michelle H.
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机构:
Obatala Sci Inc, New Orleans, LA 70148 USATulane Univ, Sch Med, New Orleans, LA 70112 USA
Ma, Michelle H.
[3
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Mohiuddin, Omair A.
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Tulane Univ, Sch Med, New Orleans, LA 70112 USATulane Univ, Sch Med, New Orleans, LA 70112 USA
Mohiuddin, Omair A.
[1
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Dietrich, Marilyn
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Louisiana State Univ, Sch Vet Med, Baton Rouge, LA 70803 USATulane Univ, Sch Med, New Orleans, LA 70112 USA
Dietrich, Marilyn
[5
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机构:
McCarthy, Michelle
[1
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Bukowska, Joanna
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机构:
Tulane Univ, Sch Med, New Orleans, LA 70112 USA
Polish Acad Sci, Inst Anim Reprod & Food Res, PL-10748 Olsztyn, PolandTulane Univ, Sch Med, New Orleans, LA 70112 USA
Bukowska, Joanna
[1
,6
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Gimble, Jeffrey M.
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机构:
Tulane Univ, Sch Med, New Orleans, LA 70112 USA
LaCell LLC, New Orleans, LA 70148 USA
Obatala Sci Inc, New Orleans, LA 70148 USATulane Univ, Sch Med, New Orleans, LA 70112 USA
Gimble, Jeffrey M.
[1
,2
,3
]
机构:
[1] Tulane Univ, Sch Med, New Orleans, LA 70112 USA
[2] LaCell LLC, New Orleans, LA 70148 USA
[3] Obatala Sci Inc, New Orleans, LA 70148 USA
[4] Axosim Inc, New Orleans, LA 70112 USA
[5] Louisiana State Univ, Sch Vet Med, Baton Rouge, LA 70803 USA
Introduction: Adipose derived stromal/stem cells (ASCs) hold potential as cell therapeutics for a wide range of disease states; however, many expansion protocols rely on the use of fetal bovine serum (FBS) as a cell culture nutrient supplement. The current study explores the substitution of lysates from expired human platelets (HPLs) as an FBS substitute. Methods: Expired human platelets from an authorized blood center were lysed by freeze/thawing and used to examine human ASCs with respect to proliferation using hematocytometer cell counts, colony forming unit-fibroblast (CFU-F) frequency, surface immunophenotype by flow cytometry, and tri-lineage (adipocyte, chondrocyte, osteoblast) differentiation potential by histochemical staining. Results: The proliferation assays demonstrated that HPLs supported ASC proliferation in a concentration dependent manner, reaching levels that exceeded that observed in the presence of 10% FBS. The concentration of 0.75% HPLs was equivalent to 10% FBS when utilized in cell culture media with respect to proliferation, immunophenotype, and CFU-F frequency. When added to osteogenic, adipogenic, and chondrogenic differentiation media, both supplements showed appropriate differentiation by staining. Conclusion: HPLs is an effective substitute for FBS in the culture, expansion and differentiation of human ASCs suitable for pre-clinical studies; however, additional assays and analyses will be necessary to validate HPLs for clinical applications and regulatory approval.
机构:
Univ Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USAUniv Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USA
Mojica-Henshaw, Mariluz P.
Jacobson, Pam
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Univ Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USAUniv Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USA
Jacobson, Pam
Morris, Julie
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机构:
Univ Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USAUniv Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USA
Morris, Julie
Kelley, Linda
论文数: 0引用数: 0
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机构:
Cryo Cell Int Inc, Oldsmar, FL USAUniv Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USA
Kelley, Linda
Pierce, Jan
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机构:
Univ Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USAUniv Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USA
Pierce, Jan
Boyer, Michael
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机构:
Univ Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USAUniv Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USA
Boyer, Michael
Reems, Jo-Anna
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机构:
Univ Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USAUniv Utah, Cell Therapy & Regenerat Med Facil, Salt Lake City, UT 84108 USA