Intranasal Vaccination with Outer-Membrane Protein of Orientia tsutsugamushi induces Protective Immunity Against Scrub Typhus

被引：6

作者：

Park, Sung-Moo ^{[1
,2
]}

Gu, Min Jeong ^{[1
,2
]}

Ju, Young-Jun ^{[1
,2
]}

Cheon, In Su ^{[1
,2
,3
]}

Hwang, Kyu-Jam ^{[4
]}

Gill, Byoungchul ^{[4
]}

Shim, Byoung-Shik ^{[3
]}

Jeong, Hang-Jin ^{[4
]}

Son, Young Min ^{[1
,2
]}

Choi, Sangho ^{[4
]}

Jeung, Woonhee ^{[1
,2
]}

Han, Seung Hyun ^{[5
,6
]}

Chu, Hyuk ^{[4
]}

Yun, Cheol-Heui ^{[1
,2
,7
,8
]}

机构：

[1] Seoul Natl Univ, Dept Agr Biotechnol, 1 Gwanak Ro, Seoul 08826, South Korea

[2] Seoul Natl Univ, Res Inst Agr & Life Sci, 1 Gwanak Ro, Seoul 08826, South Korea

[3] Int Vaccine Inst, Lab Sci Div, Seoul 08826, South Korea

[4] Natl Inst Hlth, Ctr Infect Dis Res, Div Zoonot & Vector Borne Dis Res, 187 Osongsaenginyeong 2 Ro, Cheongju 28159, South Korea

[5] Seoul Natl Univ, Sch Dent, Dept Oral Microbiol & Immunol, DRI, Seoul 08826, South Korea

[6] Seoul Natl Univ, Sch Dent, BK21 Plus Program, Seoul 08826, South Korea

[7] Seoul Natl Univ, Inst Green Bio Sci & Technol, Pyeongchang 25354, South Korea

[8] Seoul Natl Univ, Interdisciplinary Program Agr Genom, Seoul 08826, South Korea

来源：

IMMUNE NETWORK | 2021年 / 21卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Intranasal administration; Orientia tsutsugamushi; Protective immunity; Pneumonia; CONSERVED 47-KILODALTON ANTIGEN; RICKETTSIA-TSUTSUGAMUSHI; GAMMA-INTERFERON; CELL RESPONSES; TH17; CELLS; INFECTION; INDUCTION; ANTIBODY; IDENTIFICATION; IMMUNIZATION;

D O I：

10.4110/in.2021.21.e14

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Scrub typhus develops after the individual is bitten by a trombiculid mite infected with Orientia tsutsugamushi. Since it has been reported that pneumonia is frequently observed in patients with scrub typhus, we investigated whether intranasal (i.n.) vaccination with the outer membrane protein of O. tsutsugamushi (OMPOT) would induce a protective immunity against O. tsutsugamushi infection. It was particular interest that when mice were infected with O. tsutsugamushi, the bacteria disseminated into the lungs, causing pneumonia. The i.n. vaccination with OMPOT induced IgG responses in serum and bronchoalveolar lavage (BAL) fluid. The anti- O. tsutsugamushi IgA Abs in BAL fluid after the vaccination showed a high correlation of the protection against O. tsutsugamushi. The vaccination induced strong Ag-specific Th1 and Th17 responses in the both spleen and lungs. In conclusion, the current study demonstrated that i.n. vaccination with OMPOT elicited protective immunity against scrub typhus in mouse with O. tsutsugamushi infection causing subsequent pneumonia.

引用

页数：17

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