An immunosensor of B factor based on surface plasmon resonance

A novel optical immunosensor based on surface plasmon resonance (SPR) has been developed for identification of antigen. The sensor is designed on the basis of fixing angle of incidence and measuring the reflected intensities in the wavelength range of 200-800 nm. Molecular self-assembling in solution is used to form the sensing membrane on gold substrate. The kinetic processes of sensing monolayer formation were studied, Human B factor (Bf), an activator of complement C3, was detected in the concentration range from 0. 02-5 mu g/mL. Under the selected experimental conditions, the sensor has a good sensitivity, repeatability, reversibility and selectivity.