Preparation and Characterization of Silver Nanoparticle/Aloe Vera Incorporated PCL/PEO matrix for wound dressing application

Polymeric wound dressing materials have remarkable mechanical, structural, and biocompatible behavior. In this work, a polymer matrix of Polycaprolactone (PCL)/Polyethylene Oxide (PEO) incorporated with Aloe Vera (AV) extract and silver nanoparticles were prepared for wound dressing application. Initially, the phytochemicals from AV were extracted by Soxhlet apparatus, and then the aloe extract was used as a reducing agent to synthesize silver nanoparticles (Ag NP). Ag NP's formation was confirmed by the presence of a characteristic UV absorbance peak at 420 nm. Ag NP's average diameter and shape were found to be between 10-50 nm and spherical, respectively. AV extract and Ag NP were incorporated into PCL/PEO polymer solution to prepare the polymer matrix by solution casting method. Box-Behnken design (BBD) was used to study the effect of Ag NP concentration, AV extract percentage, and PEO weight percentage concerning PCL on wound dressing application. Water Vapor Transmission Rate (WVTR) and swelling properties of all the sample were tested and found that the PEO and AV extract plays a major role in both swelling and WVTR irrespective of Ag NP concentration. The antimicrobial property of synthesized Ag NP was studied against gram-negative bacteria Escherichia coli with control samples (PCL and PCL/PEO), Ag NP with 150 mg concentration showed a higher zone of inhibition than the other concentrations. Thus, the prepared PCL/PEO polymer matrix incorporated with AV extract and Ag NP can be used as an effective wound dressing material.