Signaling mediated by cell surface receptor kinases is central to the coordination of growth patterns during organogenesis. Receptor kinase signaling is in part controlled through endocytosis and subcellular distribution of the respective receptor kinase. For the majority of plant cell surface receptors, the underlying trafficking mechanisms are not characterized. In Arabidopsis, tissue morphogenesis requires the atypical receptor kinase STRUBBELIG (SUB). Here, we studied the endocytic mechanism of SUB. Our data revealed that a functional SUB-enhanced green fluorescent protein (EGFP) fusion is ubiquitinated in vivo. We further showed that plasma membrane-bound SUB:EGFP becomes internalized in a clathrin-dependent fashion. We also found that SUB: EGFP associates with the trans-Golgi network and accumulates in multivesicular bodies and the vacuole. Co-immunoprecipitation experiments revealed that SUB: EGFP and clathrin are present within the same protein complex. Our genetic analysis showed that SUB and CLATHRIN HEAVY CHAIN (CHC) 2 regulate root hair patterning. By contrast, genetic reduction of CHC activity ameliorates the floral defects of sub mutants. Taken together, the data indicate that SUB undergoes clathrin-mediated endocytosis, that this process does not rely on stimulation of SUB signaling by an exogenous agent, and that SUB genetically interacts with clathrin-dependent pathways in a tissue-specific manner.
机构:
China Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China
China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China
Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol & Dev Biol, Beijing 100101, Peoples R ChinaChina Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China
Zhao, Yan
Yan, An
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Univ Calif Riverside, Dept Bot & Sci, Ctr Plant Cell Biol, Riverside, CA 92521 USAChina Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China
Yan, An
Feijo, Jose A.
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Univ Lisboa 1700, Fac Ciencias, P-1749016 Lisbon, Portugal
Inst Gulbenkian Ciencias, P-2780156 Oeiras, PortugalChina Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China
Feijo, Jose A.
Furutani, Masahiro
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Univ Tokyo, Inst Med Sci, Dept Biochem, Minato Ku, Tokyo 1088639, JapanChina Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China
Furutani, Masahiro
Takenawa, Tadaomi
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Univ Tokyo, Inst Med Sci, Dept Biochem, Minato Ku, Tokyo 1088639, JapanChina Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China
Takenawa, Tadaomi
Hwang, Inhwan
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Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, South KoreaChina Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China
Hwang, Inhwan
Fu, Ying
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China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R ChinaChina Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China
Fu, Ying
Yang, Zhenbiao
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China Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China
Univ Calif Riverside, Dept Bot & Sci, Ctr Plant Cell Biol, Riverside, CA 92521 USAChina Agr Univ, China Agr Univ Univ Calif Riverside Joint Ctr Bio, Beijing 100193, Peoples R China