Synthesis and application of N-hydroxysuccinimidyl rhodamine B ester as an amine-reactive fluorescent probe

被引：29

作者：

Meng, Qinghua ^{[1
]}

Yu, Meijuan ^{[1
]}

Zhang, Haifeng ^{[1
]}

Ren, Jicun ^{[1
]}

Huang, Deyin ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China

来源：

DYES AND PIGMENTS | 2007年 / 73卷 / 02期

关键词：

rhodamine B; N-hydroxysuccinimidyl ester; fluorescent probe; glycine; LC-MS;

D O I：

10.1016/j.dyepig.2005.12.008

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A fluorescent probe (RB-S) containing the N-hydroxysuccinimidyl ester in 2 position was prepared from rhodamine B (RB) by one-step condensation reaction. The reactivity of the fluorescent probe with glycine was studied and the products were identified by LC-MS analysis. The excessive equivalent of the RB-S was hydrolyzed to the corresponding carboxylic acids (RB). UV/vis and fluorescence spectra of the fluorescent probe (RB-S), the labeled derivative (RB-Gly) and the hydrolysate (RB) were also studied. Fluorescence and absorption spectra appear as mirror images. The solvent effect on the spectra of the glycine derivative (RB-Gly) was investigated in methanol/water solution. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：254 / 260

页数：7

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