Clinical Significance of miR-183-3p and miR-182-5p in NSCLC and Their Correlation

被引:11
|
作者
Zhang, Tianxiang [1 ]
Li, Wei [2 ]
Gu, Meng [1 ]
Wang, Ziyu [1 ]
Zhou, Shijie [2 ]
Hao, Xuefeng [1 ]
Li, Weiying [1 ]
Xu, Shaofa [1 ]
机构
[1] Capital Med Univ, Beijing Chest Hosp, Dept Cellular & Mol Biol, Beijing TB & Thorac Tumor Res Inst, 9 Beiguan St, Beijing 101149, Peoples R China
[2] Capital Med Univ, Beijing Chest Hosp, Dept Thorac Surg, Beijing TB & Thorac Tumor Res Inst, Beijing, Peoples R China
来源
基金
中国国家自然科学基金;
关键词
miR-183-3p; miR-182-5p; non-small-cell lung cancer; NSCLC; proliferation; prognosis; CELL LUNG-CANCER; DOWN-REGULATION; PROGNOSTIC MARKER; POOR-PROGNOSIS; EXPRESSION; INVASION; ADENOCARCINOMA; PROGRESSION; MIGRATION; GROWTH;
D O I
10.2147/CMAR.S305179
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Purpose: Accumulating evidence has indicated that dysregulated microRNAs (miRNAs) are involved in cancer progression. In this study, we evaluated the clinicopathologic significance of miR-183-3p and miR-182-5p, and the role of miR-183-3p in non-small-cell lung cancer (NSCLC) progression. Patients and Methods: Seventy-six NSCLC patients from Beijing Chest Hospital were included. The expression of miR-183-3p and miR-182-5p was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR). Then, cell growth curve assays and colony formation assays were performed. Bioinformatics analysis of TCGA database was performed to explore the clinicopathological significance and prognostic value. Results: miR-183-3p and miR-182-5p were significantly increased in NSCLC tumor tissues (both P < 0.0001) and were positively correlated (r = 0.8519, P < 0.0001). miR-183-3p (P = 0.0444) and miR-182-5p (P = 0.0132) were correlated with tumor size. In addition, miR-183-3p (P = 0.0135) and miR-182-5p (P = 0.0009) were upregulated in normal lung tissues from smokers. In vitro, miR-183-3p was correlated with cell proliferation. In addition, bioinformatics analysis indicated that miR-183-3p was correlated with poor prognosis (P = 0.0466) and tumor size (P = 0.0017). In addition, miR-183-3p was higher in lung squamous carcinoma (LUSC) tissue (P < 0.0001) than in lung adenocarcinoma (LUAD) tissue, and miR-183-3p was higher in the tumor tissue of smokers (P = 0.0053) than in that of nonsmokers. Conclusion: Upregulation of miR-183-3p and miR-182-5p may play an oncogenic role in NSCLC. miR-183-3p could be used as a potential prognostic biomarker and therapeutic target to manage lung cancer.
引用
收藏
页码:3539 / 3550
页数:12
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