Two-dimensional fluorescence correlation spectroscopy

The technique and progress in two-dimensional fluorescence correlation spectroscopy is systematically introduced in the aspects of history, mathematic equations, general regulation and characteristic properties. The introduction is sorted in the range of different perturbations, such as concentration, excitation wavelength, fluorescence quenching and the pH. There are several examples in each case helping to illustrate the operability and application of the new technique. Additionally, the new technique is also compared with the traditional one-dimensional fluorescence spectroscopy, which indicates the advantage of the two-dimensional fluorescence correlation spectroscopy clearly.