Iningainema tapete sp. nov. (Scytonemataceae, Cyanobacteria) from greenhouses in central Florida (USA) produces two types of nodularin with biosynthetic potential for microcystin-LR and anabaenopeptin production

被引：8

作者：

Berthold, David E. ^{[1
]}

Lefler, Forrest W. ^{[1
]}

Huang, I-Shuo ^{[2
]}

Abdulla, Hussain ^{[3
]}

Zimba, Paul, V ^{[4
,5
]}

Laughinghouse, H. Dail ^{[1
]}

机构：

[1] Univ Florida, Ft Lauderdale Res & Educ Ctr, Dept Agron, IFAS, Davie, FL 33314 USA

[2] Virginia Inst Marine Sci, Dept Aquat Hlth Sci, Gloucester Point, VA 23062 USA

[3] Texas A&M Univ, Dept Phys & Environm Sci, Corpus Christi, TX 78412 USA

[4] Texas A&M Univ, Dept Life Sci, Corpus Christi, TX 78412 USA

[5] Texas A&M Univ, Ctr Coastal Studies, Corpus Christi, TX 78412 USA

来源：

HARMFUL ALGAE | 2021年 / 101卷

基金：

美国食品与农业研究所; 美国海洋和大气管理局;

关键词：

Cyanotoxins; Genome; Phylogeny; Taxonomy; Terrestrial; Tribrid orbitrap mass spectrometer; BALTIC SEA; TOXIN PRODUCTION; RIBOSOMAL-RNA; GENE-CLUSTER; ANATOXIN-A; WATER; GROWTH; GLYPHOSATE; ALGORITHM; HEPATOTOXINS;

D O I：

10.1016/j.hal.2020.101969

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Iningainema is a recently described genus of heterocytous, false-branching cyanobacteria originally described from Australia. In this work, we present Iningainema tapete sp. nov., isolated from subaerial and terrestrial environments in central Florida (USA). In comparison to the sister species, our novel cyanobacterium produces nodularin-R (NOD-R) and a methylated isoform [MeAdda(3)] NOD previously not reported within this genus; in addition to possessing the biosynthetic gene clusters for microcystin and anabaenopeptins production. Nodularin accumulation by this cyanobacterium exceeded 500 mu g g(-1) dry weight in cultures grown in nitrogen-depleted media. Such elevated toxin concentrations are alarming as the cyanobacterium was isolated from a food production greenhouse and poses a potential risk for food products and for workforce exposure. Using morphology, 16S rRNA gene phylogeny, and 16S-23S rRNA internal transcribed spacer (ITS) secondary structure, coupled with toxin detection and toxin gene presence, we provide evidence for the establishment of a novel toxic species of cyanobacteria, Iningainema tapete.

引用

页数：11