Label-Free Detection of DNA Hybridization Based on MnO2 Nanoparticles

被引：13
|
作者：
Liu, Zhi-Min [1 ]
Li, Zhe-Jian [1 ]
Shen, Guo-Li [2 ]
Yu, Ru-Qin [2 ]
机构：
[1] Henan Univ Technol, Coll Chem & Chem Engn, Zhengzhou 450052, Peoples R China
[2] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China
来源：
ANALYTICAL LETTERS | 2009年 / 42卷 / 18期
关键词：
DNA; electrochemical impedance spectroscopy; HIV gene; nano-MnO2; GENE SEQUENCE DETECTION; IMPEDANCE SPECTROSCOPY; MODIFIED ELECTRODE; HYDROGEN-PEROXIDE; CARBON NANOTUBES; MANGANESE OXIDES; BIOSENSOR; FILM; SENSOR;
D O I：
10.1080/00032710903276638
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Nano-MnO2/chitosan composite film modified glassy carbon electrode (MnO2/CHIT/GCE) was fabricated and a DNA probe was immobilized on the electrode surface. The immobilization and hybridization events of DNA were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The EIS was applied to the label-free detection of the target DNA. The human immunodeficiency virus (HIV) gene fragment was successfully detected by this DNA electrochemical sensor. The dynamic detection range was from 2.0x10-11 to 2.0x10-6mol/L, with a detection limit of 1.0x10-12mol/L.
引用
收藏
页码：3046 / 3057
页数：12
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