Use of deoxyribonucleoside triphosphates by mutant forms of T7 RNA polymerase

被引：0
|
作者：
Tunitskaya, VL
Memelova, LV
Kostyuk, DA
Gudima, SO
Kochetkov, SN
机构：
来源：
MOLECULAR BIOLOGY | 1997年 / 31卷 / 02期
关键词：
T7 RNA polymerase; mutant proteins; transcription; dNTP utilization; initiation site;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mutant forms of T7 RNA polymerase able to use both rNTP and dNTP as substrates were studied. The mutant Tyr639Phe,Ser641Ala can efficiently synthesize mixed polymers containing one or two deoxyribonucleotides. The mutant Tyr639Phe exhibits this property to a lower extent (no more than one deoxyribonucleotide per strand) and is practically incapable of using dGTP. The efficiency of deoxyribonucleotide utilization on the templates used in this work decreases in the order dCTP > dTTP > dATP similar to dGTP. Initiation of RNA strand synthesis is the most essential for dNTP utilization.
引用
收藏
页码：298 / 302
页数：5
相关论文
共 50 条
  • [1] Binding of Mn-deoxyribonucleoside triphosphates to the active site of the DNA polymerase of bacteriophage T7
    Akabayov, Barak
    Richardson, Charles C.
    [J]. POWDER DIFFRACTION, 2011, 26 (02) : 159 - 162
  • [2] 2 FORMS OF T7 RNA-POLYMERASE
    STRATLIN.W
    FERDINAN.FJ
    KNIPPERS, R
    [J]. HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1972, 353 (05): : 762 - &
  • [3] MUTANT T7 RNA-POLYMERASE AS A DNA-POLYMERASE
    SOUSA, R
    PADILLA, R
    [J]. EMBO JOURNAL, 1995, 14 (18): : 4609 - 4621
  • [4] Interaction of T7 RNA polymerase with affinity modifier analogs of nucleoside triphosphates
    Tunitskaya, VL
    Kochetkova, SV
    Godovikova, TS
    Kochetkov, SN
    [J]. MOLECULAR BIOLOGY, 2000, 34 (01) : 49 - 55
  • [5] T7 RNA polymerase
    Sousa, R
    Mukherjee, S
    [J]. PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 73, 2003, 73 : 1 - 41
  • [6] T7 RNA polymerase
    Rusakova, EE
    Tunitskaya, VL
    Kochetkov, SN
    [J]. MOLECULAR BIOLOGY, 1999, 33 (03) : 304 - 317
  • [7] T7 RNA Polymerase Discriminates Correct and Incorrect Nucleoside Triphosphates by Free Energy
    Wu, Shaogui
    Wang, Jiayang
    Pu, Xuemei
    Li, Laicai
    Li, Quan
    [J]. BIOPHYSICAL JOURNAL, 2018, 114 (08) : 1755 - 1761
  • [8] Synthesis of mixed ribo/deoxyribopolynucleotides by mutant T7 RNA polymerase
    Gudima, SO
    Kostyuk, DA
    Grishchenko, OI
    Tunitskaya, VL
    Memelova, LV
    Kochetkov, SN
    [J]. FEBS LETTERS, 1998, 439 (03) : 302 - 306
  • [9] PROCESSIVITY OF PROTEOLYTICALLY MODIFIED FORMS OF T7 RNA-POLYMERASE
    MULLER, DK
    MARTIN, CT
    COLEMAN, JE
    [J]. BIOCHEMISTRY, 1988, 27 (15) : 5763 - 5771
  • [10] Synthesis of mixed ribo/deoxyribopolynucleotides by mutant T7 RNA polymerase.
    Tunitskaya, VL
    Gudima, SO
    Kostyuk, DA
    Kochetkov, SN
    [J]. NUCLEOSIDES & NUCLEOTIDES, 1999, 18 (6-7): : 1239 - 1240
12345