Quantification of hepatitis C virus in human liver and serum samples by using lightcycler reverse transcriptase PCR

被引：40
|
作者：
White, PA
Pan, Y
Freeman, AJ
Marinos, G
Ffrench, RA
Lloyd, AR
Rawlinson, WD
机构：
[1] Prince Wales Hosp, SEALS, Dept Microbiol, Div Virol, Sydney, NSW 2031, Australia
[2] Prince Wales Hosp, Viral Hepatitis Res Gastrointestinal & Liver Unit, Sydney, NSW 2031, Australia
[3] Sydney Childrens Hosp, Dept Immunol & Infect Dis, Sydney, NSW 2031, Australia
[4] Univ New S Wales, Fac Sci, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia
[5] Univ New S Wales, Fac Med, Sch Med Sci, Sydney, NSW 2052, Australia
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 11期
关键词：
D O I：
10.1128/JCM.40.11.4346-4348.2002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A highly sensitive, non-probe-based, real-time quantitative reverse transcriptase PCR was developed for viral load measurements in both serum and liver samples from patients with hepatitis C virus (HCV) infection. With synthetic RNA, the linearity of the approach was conserved over a wide range of HCV copy numbers. There was a strong correlation between hepatic and serum viral load measurements (r = 0.689, P = 0.004, n = 15), indicating that the level of viremia reflected the amount of virus present in the liver.
引用
收藏
页码：4346 / 4348
页数：3
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