Characterization of a dammarenediol synthase in Centella asiatica (L.) Urban

被引：24

作者：

Kim, Ok Tae ^{[1
]}

Lee, Jei Wan ^{[1
]}

Bang, Kyong Hwan ^{[1
]}

Kim, Young Chang ^{[1
]}

Hyun, Dong Yun ^{[1
]}

Cha, Seon Woo ^{[1
]}

Choi, Yong Eui ^{[2
]}

Jin, Mei Lan ^{[3
]}

Hwang, Baik ^{[3
]}

机构：

[1] RDA, Natl Inst Hort & Herbal Sci, Dept Herbal Crop Res, Eumseong 369871, South Korea

[2] Kangwon Natl Univ, Coll Forest Sci, Forest Resources Div, Chunchon 200701, South Korea

[3] Chonnam Natl Univ, Dept Biol, Kwangju 500757, South Korea

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2009年 / 47卷 / 11-12期

关键词：

Dammarenediol synthase; Centella asiatica; Oxidosqualene cyclase; BETA-AMYRIN SYNTHASE; OXIDOSQUALENE CYCLASE; CYCLOARTENOL SYNTHASE; LUPEOL SYNTHASE; TRITERPENE; BIOSYNTHESIS; CLONING; EXPRESSION; ENZYME; GENE;

D O I：

10.1016/j.plaphy.2009.08.001

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

To elucidate the exact function of CabAS in Centella asiatica, which was previously reported as a putative beta-amyrin synthase [Plant Cell Rep, 24:304-311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). After inducing the CabAS gene with galactose, a peak consistent with the dammarenediol standard was detected in LC/APCIMS analyses and the accumulated product was confirmed as dammarenediol. CabAS should therefore be renamed to C asiatica dammarenediol synthase (CaDDS). The confirmation of this gene function may allow us to better understand the generation of numerous triterpene carbon skeletons. (C) 2009 Elsevier Masson SAS. All rights reserved.

引用

页码：998 / 1002

页数：5

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