Development and validation of a competitive ELISA kit for the serological diagnosis of ovine, caprine and bovine brucellosis

被引：16
|
作者：
Portanti, O. [1 ]
Tittarelli, M. [1 ]
Di Febo, T. [1 ]
Luciani, M. [1 ]
T Mercante, M. [1 ]
Conte, A. [1 ]
Lelli, R. [1 ]
机构：
[1] Ist Zooprofilatt Sperimentale Abruzzo & Molise G, I-64100 Teramo, Italy
来源：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH | 2006年 / 53卷 / 10期
关键词：
D O I：
10.1111/j.1439-0450.2006.00971.x
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A competitive ELISA (Brucella-Ab c-ELISA) was standardized and validated for the detection of Brucella antibodies in cattle, sheep and goat sera using a monoclonal antibody (MAb 4B5A) produced against Brucella melitensis biotype 2. The specificity and sensitivity of the assay were 100% to a 67.5% cut-off point (B/Bo%). When compared with an indirect ELISA, the Brucella-Ab c-ELISA did not demonstrate cross-reactions when testing positive sera for antibodies to some Enterobacteriaceae. A comparison was made between the Brucella-Ab c-ELISA and the complement fixation and Rose Bengal tests. Results demonstrated that the Brucella-Ab c-ELISA is a valuable tool for the serological diagnosis of bovine and ovine/caprine brucellosis.
引用
收藏
页码：494 / 498
页数：5
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