Average Conformation of Branched Chain Lipid PGP-Me That Accounts for the Thermal Stability and High-Salinity Resistance of Archaeal Membranes

被引：7

作者：

Yamagami, Masaki ^{[1
,2
]}

Tsuchikawa, Hiroshi ^{[1
]}

Cui, Jin ^{[1
,2
]}

Umegawa, Yuichi ^{[1
,2
]}

Miyazaki, Yusuke ^{[3
]}

Seo, Sangjae ^{[3
]}

Shinoda, Wataru ^{[3
]}

Murata, Michio ^{[1
,2
]}

机构：

[1] Osaka Univ, Grad Sch Sci, Dept Chem, 1-1 Machikaneyama, Toyonaka, Osaka 5600043, Japan

[2] Osaka Univ, Lipid Act Struct Project, JST ERATO, 1-1 Machikaneyama, Toyonaka, Osaka 5600043, Japan

[3] Nagoya Univ, Grad Sch Engn, Dept Chem Mat, Nagoya, Aichi 4648603, Japan

来源：

BIOCHEMISTRY | 2019年 / 58卷 / 37期

关键词：

MOLECULAR-DYNAMICS SIMULATION; THERMOTROPIC PHASE-BEHAVIOR; PURPLE MEMBRANE; FATTY-ACIDS; PHYSICAL-PROPERTIES; MAGNETIC-RESONANCE; EXTREME HALOPHILES; FORCE-FIELD; PROTON PUMP; BACTERIORHODOPSIN;

D O I：

10.1021/acs.biochem.9b00469

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The average conformation of the methyl-branched chains of archaeal lipid phosphatidyl glycerophosphate methyl ester (PGP-Me) was examined in a hydrated bilayer membrane based on the H-2 nuclear magnetic resonance (NMR) of enantioselectively H-2-labeled compounds that were totally synthesized for the first time in this study. The NMR results in combination with molecular dynamics simulations revealed that the PGP-Me chain appeared to exhibit behavior different from that of typical membrane lipids such as dimyristoylphosphatidylcholine (DMPC). The C-C bonds of the PGP-Me chain adopt alternative parallel and tilted orientations to the membrane normal as opposed to a DMPC chain where all of the C-C bonds tilt in the same way on average. This characteristic orientation causes the intertwining of PGP-Me chains, which plays an important role in the excellent thermal and high-salinity stabilities of archaeal lipid bilayers and membrane proteins.

引用

页码：3869 / 3879

页数：11