Isolation of polymorphic microsatellite loci from the flea beetle Phyllotreta nemorum L. (Coleoptera: Chrysomelidae)

被引：1
|
作者：
Verbaarschot, P.
Calvo, D.
Esselink, G. D.
Molina, Ma.
Vrieling, K.
De Jong, P. W.
机构：
[1] Univ Wageningen & Res Ctr, Entomol Lab, NL-6700 EH Wageningen, Netherlands
[2] IFAPA, CIFA Las Torres Tomejil, CICE, Seville, Spain
[3] Wageningen UR, Biodivers & Breeding, Plant Res Int, NL-6700 AA Wageningen, Netherlands
[4] Leiden Univ, Sect Ecol, Inst Biol, NL-2300 RA Leiden, Netherlands
来源：
MOLECULAR ECOLOGY NOTES | 2007年 / 7卷 / 01期
关键词：
Barbarea vulgaris; Denmark; flea beetle; microsatellites;
D O I：
10.1111/j.1471-8286.2006.01524.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Ten microsatellite markers for the flea beetle Phyllotreta nemorum were developed using di- and trinucleotide repeat-enriched libraries. Each of these primer pairs were characterized on 96 individuals. Expected heterozygosities ranged between 0.11 and 0.84 and the number of alleles ranged between two and 14 per locus. These microsatellite markers are the first published for any Phyllotreta species.
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页码：60 / 62
页数：3
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