Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding

被引:0
|
作者
Rodriguez, V
Llambí, S
Postiglioni, A
Guevara, K
Rincón, G
Fernández, G
Mernies, B
Arruga, MV
机构
[1] UDELAR, Fac Vet, Dept Cell & Mol Biol, Genet Sect,Lab Genet Anal Domest Anim, Montevideo 11600, Uruguay
[2] UDELAR, Fac Vet, Dept Anim Prod, Anim Breeding Sect, Montevideo, Uruguay
[3] Univ Zaragoza, Fac Vet, Lab Cytogenet & Mol Genet, E-50009 Zaragoza, Spain
关键词
cattle; chromosome; fragile sites; aphidicolin;
D O I
10.1186/1297-9686-34-6-649
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Fragile sites (FS) seem to play a role in genome instability and may be involved in karyotype evolution and chromosome aberrations. The majority of common fragile sites are induced by aphidicolin. Aphidicolin was used at two different concentrations (0.15 and 0.30 muM) to study the occurrence of FS in the cattle karyotype. In this paper, a map of aphidicolin induced break points and fragile sites in cattle chromosomes was constructed. The statistical analysis indicated that any band with three or more breaks was significantly damaged (P < 0.05). According to this result, 30 of the 72 different break points observed were scored as fragile sites. The Pearson correlation test showed a positive association between chromosome length and the number of fragile sites (r = 0.54). On the contrary, 21 FS were identified on negative R bands while 9 FS were located on positive R bands.
引用
收藏
页码:649 / 656
页数:8
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    Karina Guevara
    Gonzalo Rincón
    Gabriel Fernández
    Beatriz Mernies
    María Victoria Arruga
    [J]. Genetics Selection Evolution, 34
  • [2] LOCALIZATION OF THE FRAGILE-X CHROMOSOME BREAK POINTS IN HOLSTEIN-FRIESIAN CATTLE (BOS-TAURUS)
    LLAMBI, S
    POSTIGLIONI, A
    [J]. THERIOGENOLOGY, 1994, 42 (05) : 789 - 794
  • [3] CILIATED PROTOZOA IN THE RUMEN OF HOLSTEIN-FRIESIAN CATTLE (BOS-TAURUS-TAURUS) IN HOKKAIDO, JAPAN, WITH THE DESCRIPTION OF 2 NEW SPECIES
    ITO, A
    IMAI, S
    [J]. ZOOLOGICAL SCIENCE, 1990, 7 (03) : 449 - 458
  • [4] Associations between novel single nucleotide polymorphisms in the Bos taurus growth hormone gene and performance traits in Holstein-Friesian dairy cattle
    Mullen, M. P.
    Berry, D. P.
    Howard, D. J.
    Diskin, M. G.
    Lynch, C. O.
    Berkowicz, E. W.
    Magee, D. A.
    MacHugh, D. E.
    Waters, S. M.
    [J]. JOURNAL OF DAIRY SCIENCE, 2010, 93 (12) : 5959 - 5969
  • [5] Associations between novel single nucleotide polymorphisms in the Bos taurus growth hormone gene and performance traits in Holstein-Friesian dairy cattle (vol 93, pg 5959, 2010)
    Mullen, M. P.
    Berry, D. P.
    Howard, D. J.
    Diskin, M. G.
    Lynch, C. O.
    Berkowicz, E. W.
    Magee, D. A.
    MacHugh, D. E.
    Waters, S. M.
    [J]. JOURNAL OF DAIRY SCIENCE, 2011, 94 (02) : 1069 - 1069