Extender supplementation with catalase maintains the integrity of sperm plasma membrane after freezing-thawing of semen from capuchin monkey

被引:5
|
作者
Leao, Danuza L. [2 ]
Brito, Adriel B. [2 ]
Miranda, Stefania A. [2 ]
Oliveira, Karol G. [1 ,2 ]
Almeida, Debora V. C. [2 ]
Santos, Regiane R.
Domingues, Sheyla F. S. [2 ]
机构
[1] Natl Primate Ctr, Ananindeua, PA, Brazil
[2] Fed Univ Para, Fac Vet Med, Lab Amazon Anim Biotechnol & Med, Belem, Para, Brazil
关键词
Antioxidant; Cell membrane; Monkey; Semen; SQUIRREL-MONKEY; HUMAN SPERMATOZOA; CRYOPRESERVATION; INFERTILITY;
D O I
10.1017/S0967199416000447
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We aimed to evaluate the effect of supplementation of ACP-118((R)) extender with the antioxidant catalase (10 and 50 mu g/ml) on Sapajus apella sperm motility, vigour, and plasma membrane integrity during the processes of seminal liquefaction, cooling, and freezing. Catalase did not affect any of the evaluated parameters after semen dilution or cooling. Cryopreserved sperm in the presence of 50 mu g/ml catalase presented a plasma membrane integrity similar to that fresh sperm, however.
引用
收藏
页码:231 / 234
页数:4
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