Bacteriophage as an Alternative to Antibiotics Promotes Growth Performance by Regulating Intestinal Inflammation, Intestinal Barrier Function and Gut Microbiota in Weaned Piglets

被引:35
|
作者
Zeng, Yongdi [1 ,2 ]
Wang, Zirui [1 ,2 ]
Zou, Tiande [1 ,2 ]
Chen, Jun [1 ,2 ]
Li, Guanhong [1 ,2 ]
Zheng, Liuzhen [1 ,2 ]
Li, Shuo [1 ,2 ]
You, Jinming [1 ,2 ]
机构
[1] Jiangxi Agr Univ, Jiangxi Prov Key Lab Anim Nutr, Nanchang, Jiangxi, Peoples R China
[2] Jiangxi Agr Univ, Jiangxi Prov Key Innovat Ctr Integrat Prod & Educ, Nanchang, Jiangxi, Peoples R China
关键词
antibiotic-free diet; bacteriophage; diarrhea incidence; growth performance; gut microbiota; intestinal barrier function; intestinal inflammation; ZINC-OXIDE; DIETARY SUPPLEMENTATION; ANTIOXIDANT SYSTEM; IMMUNE-RESPONSES; PERMEABILITY; EXPRESSION; MORPHOLOGY; DIARRHEA; HEALTH; TIGHT;
D O I
10.3389/fvets.2021.623899
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
This study aimed to investigate the effects of dietary bacteriophage supplementation on growth performance, intestinal morphology, barrier function, and intestinal microbiota of weaned piglets fed antibiotic-free diet. A total of 120 weaned piglets were allotted to four dietary treatments with five pens/treatment and six piglets/pen in a 21-d feeding trial. The control diet was supplemented with 25 mg/kg quinocetone and 11.25 mg/kg aureomycin in the basal diet, while the three treatment diets were supplemented with 200, 400, or 600 mg/kg bacteriophage in the basal diet, respectively. There was no difference for growth performance and all measured indices of serum and intestinal tissues between 200 mg/kg bacteriophage group and the control group with antibiotics (P > 0.05). More importantly, compared with the control diet, dietary 400 mg/kg bacteriophage inclusion increased average daily gain and average daily feed intake, and decreased feed/gain ratio and diarrhea incidence of weaned piglets (P < 0.05). Also, piglets fed 400 mg/kg bacteriophage had elevated villi height (VH) in jejunum and ileum, reduced crypt depth (CD) in jejunum and ileum, and elevated VH/CD ratio in duodenum, jejunum and ileum (P < 0.05). Compared to the control group, piglets fed 400 mg/kg bacteriophage had lower interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha), and higher interleukin-10 (IL-10) concentration in serum, and higher secretory immunoglobulin A (sIgA), intestinal trefoil factor (ITF), and tumor growth factor-alpha (TGF-alpha) content in the ileal mucosa (P < 0.05). Besides, dietary addition with 400 mg/kg bacteriophage decreased the D-lactate concentration and diamine oxidase (DAO) activity in serum, and increased the relative mRNA expression of ZO-1, Claudin-1, Occludin, TLR2, TLR4, and TLR9, as well as the relative protein expression of Occludin in the jejunum (P < 0.05). However, the growth performance and all analyzed parameters in serum and intestinal tissues were not further improved when piglets fed 600 vs. 400 mg/kg bacteriophage (P > 0.05). MiSeq sequencing analysis showed that bacteriophage regulated the microbial composition in caecum digesta, as indicated by higher observed_species, Chao1, and ACE richness indices, as well as changes in the relative abundance of Firmicutes, Bacteroidetes, and Tenericutes (P < 0.05). Collectively, 400 mg/kg bacteriophage can be used as an antibiotics alternative for promoting the growth of weaned piglets. The underlying mechanism is associated with a positive effect of bacteriophage on intestinal inflammation, intestinal barrier function and gut microbiota in weaned piglets.
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页数:14
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