Effect of loureirin A against Candida albicans biofilms

被引:22
|
作者
Lin Mei-Yu [1 ]
Yuan Zhong-Lan [1 ]
Hu Dan-Dan [1 ]
Hu Gan-Hai [1 ,2 ]
Zhang Ri-Li [1 ]
Zhong Hua [1 ]
Yan Lan [1 ]
Jiang Yuan-Ying [1 ]
Su Juan [1 ]
Wang Yan [1 ]
机构
[1] Naval Mil Med Univ, Sch Pharm, Shanghai 200433, Peoples R China
[2] Fujian Univ Tradit Chinese Med, Dept Pharm, Fuzhou 350000, Fujian, Peoples R China
基金
中国国家自然科学基金;
关键词
Candida albicans; Loureirin A; Anti-biofilm; Morphological transition; CELL-SURFACE HYDROPHOBICITY; NOSOCOMIAL FUNGAL-INFECTIONS; GALLERIA-MELLONELLA; HYPHAL FORMATION; IN-VITRO; ADHERENCE; EPIDEMIOLOGY; SUPPRESSION; PATHOGEN; MUTANTS;
D O I
10.1016/S1875-5364(19)30064-0
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Loureirin A is a major active component of Draconis sanguis, a traditional Chinese medicine. This work aimed to investigate the activity of loureirin A against Candida albicans biofilms. 2, 3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay and scanning electron microscopy were used to investigate the anti-biofilm effect. Minimal inhibitory concentration testing and time-kill curve assay were used to evaluate fungicidal activity. Cell surface hydrophobicity (CSH) assay and hyphal formation experiment were respectively carried out to investigate adhesion and morphological transition, two virulence traits of C. albicans. Real-time RT-PCR was used to investigate gene expression. Galleria mellonella-C. albicans and Caenorhabditis elegans-C. albicans infection models were used to evaluate the in-vivo antifungal effect. Human umbilical vein endothelial cells and C. elegans nematodes were used to evaluate the toxicity of loureirin A. Our data indicated that loureirin A had a significant effect on inhibiting C. albicans biofilms, decreasing CSH, and suppressing hyphal formation. Consistently, loureirin A down-regulated the expression of some adhesion-related genes and hypha/biofilm-related genes. Moreover, loureirin A prolonged the survival of Galleria mellonella and Caenorhabditis elegans in C. albicans infection models and exhibited low toxicity. Collectively, loureirin A inhibits fungal biofilms, and this effect may be associated with the suppression of pathogenic traits, adhesion and hyphal formation.
引用
收藏
页码:616 / 623
页数:8
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