Supply Chain Agility Assessment has been considered as one of the most important issues in world class manufacturing research study. The environmental issue as well as speed, variety, flexibility and integration in production line come along with customer relationship and mass customization concept should come to light in today's research study. To achieve the competitive edge, companies must align with suppliers and customers to streamline operations, as well as agility characteristic beyond individual companies. Consequently agile supply chain (ASC) is considered as a dominant competitive advantage. However, so far a little effort has been made to build ASC assessment methodology in recent years. Therefore, due to ambiguity of agility assessment, we have constructed a fuzzy rule based system, considering agility capabilities such as Responsiveness, competency, flexibility and Quickness to evaluate agility in supply chains. This evaluation helps managers to perform gap analysis between existent agility level and the desired one.