Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

被引：43
|
作者：
Amer, Said [1 ,2 ]
Helal, Ibrahim B. [3 ]
Kamau, Evelyne [4 ]
Feng, Yaoyu [5 ]
Xiao, Lihua [1 ]
机构：
[1] Ctr Dis Control & Prevent, Natl Ctr Emerging Zoonot Infect Dis, Div Foodborne Waterborne & Environm Dis, Atlanta, GA USA
[2] Kafr El Sheikh Univ, Fac Sci, Dept Zool, Kafr Al Sheikh, Egypt
[3] Tanta Univ, Fac Sci, Dept Zool, Tanta, Egypt
[4] Univ Evry Val Essonne, ISSB, Evry, France
[5] E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
PLOS ONE | 2015年 / 10卷 / 03期
基金：
中国国家自然科学基金;
关键词：
HUMAN CYSTIC ECHINOCOCCOSIS; IDENTIFICATION; STRAINS; EPIDEMIOLOGY; HUMANS; SHEEP; DOGS; G1;
D O I：
10.1371/journal.pone.0118509
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Little is known on the diversity and public health significance of Echinococcus species in livestock in Egypt. In this study, 37 individual hydatid cysts were collected from dromedary camels (n=28), sheep (n=7) and buffalos (n=2). DNA was extracted from protoscoleces/germinal layer of individual cysts and amplified by PCR targeting nuclear (actin II) and mitochondrial (COX1 and NAD1) genes. Direct sequencing of amplicons indicated the presence of Echinococcus canadenesis (G6 genotype) in 26 of 28 camel cysts, 3 of 7 sheep cysts and the 2 buffalo derived cysts. In contrast, Echinococcus granulosus sensu stricto (G1 genotype) was detected in one cyst from a camel and 4 of 7 cysts from sheep, whereas Echinococcus ortleppi (G5 genotype) was detected in one cyst from a camel. This is the first identification of E. ortleppi in Egypt.
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页数：12
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