A safe and effective vaccine against bovine leukemia virus

被引:11
|
作者
Suarez Archilla, Guillermo [1 ]
Gutierrez, Geronimo [2 ]
Camussone, Cecilia [1 ]
Calvinho, Luis [1 ]
Abdala, Alejandro [1 ]
Alvarez, Irene [2 ]
Petersen, Marcos [2 ]
Franco, Lautaro [2 ]
Destefano, Gabriel [2 ]
Monti, Gustavo [3 ]
Jacques, Jean-Rock [4 ,5 ]
Joris, Thomas [4 ,5 ]
Willems, Luc [4 ,5 ]
Trono, Karina [2 ]
机构
[1] Inst Invest Cadena Lactea INTA CONICET, Estn Expt Agr Rafaela, Rafaela, Argentina
[2] Ctr Invest Ciencias Vet & Agron INTA CONICET, Inst Virol Innovac Tecnol, Hurlingham, Argentina
[3] Wageningen Univ & Res, Quantitat Vet Epidemiol Grp, Wageningen, Netherlands
[4] Univ Liege ULiege, Mol & Cellular Epigenet GIGA & Mol Biol TERRA, Liege, Belgium
[5] GIGA Univ Liege ULiege, Mol & Cellular Epigenet, Interdisciplinary Cluster Appl Genoprote GIGA, Liege, Belgium
来源
FRONTIERS IN IMMUNOLOGY | 2022年 / 13卷
关键词
BLV; vaccine; attenuated strain; retrovirus; HTLV; IN-VIVO INFECTION; DAIRY; TRANSMISSION; ANTIBODIES; CATTLE; BLOOD; SHEEP;
D O I
10.3389/fimmu.2022.980514
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Previous attempts to develop a vaccine against bovine leukemia virus (BLV) have not been successful because of inadequate or short-lived stimulation of all immunity components. In this study, we designed an approach based on an attenuated BLV provirus by deleting genes dispensable for infectivity but required for efficient replication. The ability of the vaccine to protect from natural BLV infection was investigated in the context of dairy productive conditions in an endemic region. The attenuated vaccine was tested in a farm in which the prevalence rose from 16.7% in young cattle at the beginning of the study to more than 90% in adult individuals. Sterilizing immunity was obtained in 28 out of 29 vaccinated heifers over a period of 48 months, demonstrating the effectiveness of the vaccine. As indicated by the antiviral antibody titers, the humoral response was slightly reduced compared to wild-type infection. After initial post-vaccination bursts, the proviral loads of the attenuated vaccine remained most frequently undetectable. During the first dairy cycle, proviral DNA was not detected by nested-PCR in milk samples from vaccinated cows. During the second dairy cycle, provirus was sporadically detected in milk of two vaccinated cows. Forty-two calves born from vaccinated cows were negative for proviral DNA but had antiviral antibodies in their peripheral blood. The attenuated strain was not transmitted to sentinels, further supporting the safety of the vaccine. Altogether, these data thus demonstrate that the vaccine against BLV is safe and effective in herd conditions characterized by a very high incidence. This cost-effective approach will thus decrease the prevalence of BLV without modification of production practices. After facing a series of challenges pertaining to effectiveness and biosafety, the vaccine is now available for further large-scale delivery. The different challenges and hurdles that were bypassed may be informative for the development of a vaccine against HTLV-1.
引用
收藏
页数:9
相关论文
共 50 条
  • [1] An efficient vaccine against bovine leukemia virus
    Gutierrez, Geronimo
    Rodriguez, Sabrina M.
    Vilor, Agustin
    Gillet, Nicolas
    DeBrogniez, Alix
    Trono, Karina
    Willems, Luc
    [J]. RETROVIROLOGY, 2015, 12
  • [2] An efficient vaccine against bovine leukemia virus
    Gerónimo Gutiérrez
    Sabrina M Rodríguez
    Agustín Vilor
    Nicolas Gillet
    Alix DeBrogniez
    Karina Trono
    Luc Willems
    [J]. Retrovirology, 12
  • [3] Generation of virus-like particle as a vaccine strategy against bovine leukemia virus
    Otsuki, Hiroyuki
    Takeshima, Shin-nosuke
    Aida, Yoko
    [J]. RETROVIROLOGY, 2015, 12
  • [4] Generation of virus-like particle as a vaccine strategy against bovine leukemia virus
    Hiroyuki Otsuki
    Shin-nosuke Takeshima
    Yoko Aida
    [J]. Retrovirology, 12
  • [5] CELL-DERIVED VACCINE AGAINST BOVINE LEUKEMIA-VIRUS INFECTION
    ALTANER, C
    ALTANEROVA, V
    BAN, J
    JANIK, V
    VOLEJNICEK, V
    FRAJS, Z
    CERNY, L
    [J]. JOURNAL OF VETERINARY MEDICINE SERIES B-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1988, 35 (10): : 736 - 746
  • [6] Bovine leukemia virus and human detection of antibody against Bovine Leukemia Virus
    Pourjafar, M.
    Mahzounieh, M.
    Falah, A.
    [J]. ANNALS OF ONCOLOGY, 2008, 19 : 202 - 203
  • [7] Vaccine against ricin proves safe and effective
    不详
    [J]. EXPERT REVIEW OF VACCINES, 2006, 5 (02) : 173 - 173
  • [8] Finding a safe and effective vaccine for the Lassa virus
    Okokhere, Peter O.
    [J]. LANCET, 2023, 401 (10384): : 1242 - 1243
  • [9] Safe, effective, recombinant subunit vaccine for protection against dengue virus induced disease
    Coller, Beth-Ann
    Lieberman, Michael
    Putnak, J. Robert
    Clements, David
    Ogata, Steven
    Thorne, Michael
    Martyak, Timothy
    Chang, David
    Lehrer, Axel
    Wong, Teri
    Weeks-Levy, Carolyn
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2006, 75 (05): : 281 - 281
  • [10] A recombinant attenuated candidate vaccine that efficiently and persistently protects against bovine leukemia virus in herds
    Sabrina M Rodríguez
    Gerónimo Gutiérrez
    Nicolas Gillet
    Karina Trono
    Luc Willems
    [J]. Retrovirology, 11 (Suppl 1)