Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells

被引:254
|
作者
Ezratty, Ellen J. [1 ]
Bertaux, Claire [1 ]
Marcantonio, Eugene E. [1 ]
Gundersen, Gregg G. [1 ]
机构
[1] Columbia Univ, Dept Pathol & Cell Biol, New York, NY 10032 USA
来源
JOURNAL OF CELL BIOLOGY | 2009年 / 187卷 / 05期
基金
美国国家卫生研究院;
关键词
COATED PITS; CYTOPLASMIC DOMAIN; DISABLED-2; DAB2; DYNAMICS; MOTILITY; KINASE; CALCINEURIN; FIBROBLASTS; NEUTROPHILS; INITIATION;
D O I
10.1083/jcb.200904054
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Focal adhesion disassembly is regulated by microtubules (MTs) through an unknown mechanism that involves dynamin. To test whether endocytosis may be involved, we interfered with the function of clathrin or its adaptors autosomal recessive hypercholesteremia (ARH) and Dab2 (Disabled-2) and found that both treatments prevented MT-induced focal adhesion disassembly. Surface labeling experiments showed that integrin was endocytosed in an extra-cellular matrix-, clathrin-, and ARH- and Dab2-dependent manner before entering Rab5 endosomes. Clathrin colocalized with a subset of focal adhesions in an ARH- and Dab2-dependent fashion. Direct imaging showed that clathrin rapidly accumulated on focal adhesions during MT-stimulated disassembly and departed from focal adhesions with integrin upon their disassembly. In migrating cells, depletion of clathrin or Dab2 and ARH inhibited focal adhesion disassembly and decreased the rate of migration. These results show that focal adhesion disassembly occurs through a targeted mechanism involving MTs, clathrin, and specific clathrin adaptors and that direct endocytosis of integrins from focal adhesions mediates their disassembly in migrating cells.
引用
收藏
页码:733 / 747
页数:15
相关论文
共 50 条
  • [1] Focal adhesion disassembly requires clathrin-dependent endocytosis of integrins
    Chao, Wei-Ting
    Kunz, Jeannette
    [J]. FEBS LETTERS, 2009, 583 (08): : 1337 - 1343
  • [2] PDK1 regulates focal adhesion disassembly by modulating endocytosis of αvβ3 integrin
    di Blasio, Laura
    Gagliardi, Paolo Armando
    Puliafito, Alberto
    Sessa, Roberto
    Seano, Giorgio
    Bussolino, Federico
    Primo, Luca
    [J]. JOURNAL OF CELL SCIENCE, 2015, 128 (05) : 863 - 877
  • [3] Septins guide noncentrosomal microtubules to promote focal adhesion disassembly in migrating cells
    Merenich, Daniel
    Nakos, Konstantinos
    Pompan, Taylor
    Donovan, Samantha J.
    Gill, Amrik
    Patel, Pranav
    Spiliotis, Elias T.
    Myers, Kenneth A.
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2022, 33 (05)
  • [4] Nanomanipulation of Ligand Nanogeometry Modulates Integrin/Clathrin-Mediated Adhesion and Endocytosis of Stem Cells
    Yin, Bohan
    Zhang, Qin
    Yan, Jiaxiang
    Huang, Yingying
    Li, Chuanqi
    Chen, Jiareng
    Wen, Chunyi
    Wong, Siu Hong Dexter
    Yang, Mo
    [J]. NANO LETTERS, 2023, 23 (19) : 9160 - 9169
  • [5] Type I Phosphatidylinositol Phosphate Kinase Beta Regulates Focal Adhesion Disassembly by Promoting β1 Integrin Endocytosis
    Chao, Wei-Ting
    Ashcroft, Felicity
    Daquinag, Alexes C.
    Vadakkan, Tegy
    Wei, Zhubo
    Zhang, Pumin
    Dickinson, Mary E.
    Kunz, Jeannette
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2010, 30 (18) : 4463 - 4479
  • [6] Integrin endocytosis at the leading edge of protrusions in migrating cells
    Webb, DJ
    Laukaitis, CM
    Horwitz, AR
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2000, 11 : 85A - 86A
  • [7] Asymmetric focal adhesion disassembly in motile cells
    Broussard, Joshua A.
    Webb, Donna J.
    Kaverina, Irina
    [J]. CURRENT OPINION IN CELL BIOLOGY, 2008, 20 (01) : 85 - 90
  • [8] Clathrin mediated endocytosis polarized away from the lagging edge migrating cells
    Rappoport, JZ
    Simon, SM
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2002, 13 : 470A - 470A
  • [9] Binding of APC and dishevelled mediates Wnt5a-regulated focal adhesion dynamics in migrating cells
    Matsumoto, Shinji
    Fumoto, Katsumi
    Okamoto, Tetsuji
    Kaibuchi, Kozo
    Kikuchi, Akira
    [J]. EMBO JOURNAL, 2010, 29 (07): : 1192 - 1204
  • [10] Thrombospondin mediates focal adhesion disassembly through interactions with cell surface calreticulin
    Goicoechea, S
    Orr, AW
    Pallero, MA
    Eggleton, P
    Murphy-Ullrich, JE
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (46) : 36358 - 36368