The proton permeability of liposomes made from mitochondrial inner membrane phospholipids: Comparison with isolated mitochondria

被引:84
|
作者
Brookes, PS
Rolfe, DFS
Brand, MD
机构
[1] Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, Tennis Court Road
来源
JOURNAL OF MEMBRANE BIOLOGY | 1997年 / 155卷 / 02期
关键词
mitochondria; vesicle; proton leak; phospholipid; liposome; fatty acid;
D O I
10.1007/s002329900168
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Unilamellar liposomes with native phospholipid fatty acid composition were prepared from rat liver mitochondrial inner membrane phospholipids by extrusion in medium containing 50 mM potassium. They were diluted into low potassium medium to establish a transmembrane potassium gradient. A known membrane potential was imposed by addition of valinomycin, and proton flux into liposomes was measured. Valinomycin in the range 10 pM-1nM was sufficient to fully establish membrane potential. Valinomycin concentrations above 3 nn? catalyzed additional proton flux and were avoided. At 300 pM valinomycin, proton flux depended nonlinearly on membrane potential. At 160 mV membrane potential the flux was 30 nmol H+/min/mg phospholipid-approximately 5% of the proton leak flux under comparable conditions in isolated mitochondria, indicating that leak pathways through bulk phospholipid bilayer account for only a small proportion of total mitochondrial proton leak.
引用
收藏
页码:167 / 174
页数:8
相关论文
共 50 条
  • [1] The Proton Permeability of Liposomes Made from Mitochondrial Inner Membrane Phospholipids: Comparison with Isolated Mitochondria
    P.S. Brookes
    D.F.S. Rolfe
    M.D. Brand
    [J]. The Journal of Membrane Biology , 1997, 155 : 167 - 174
  • [2] The proton permeability of liposomes made from mitochondrial inner membrane phospholipids: no effect of fatty acid composition
    Brookes, PS
    Hulbert, AJ
    Brand, MD
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1330 (02): : 157 - 164
  • [3] The Mechanisms of Fatty Acid-Induced Proton Permeability of the Inner Mitochondrial Membrane
    Lech Wojtczak
    Mariusz R. Wie¸ckowski
    [J]. Journal of Bioenergetics and Biomembranes, 1999, 31 : 447 - 455
  • [4] The mechanisms of fatty acid-induced proton permeability of the inner mitochondrial membrane
    Wojtczak, L
    Wieckowski, MR
    [J]. JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1999, 31 (05) : 447 - 455
  • [5] PERMEABILITY OF MITOCHONDRIAL INNER MEMBRANE TO SUCROSE
    GAMBLE, JL
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 211 (02) : 223 - +
  • [6] PERMEABILITY OF INNER MEMBRANE OF MITOCHONDRIA TO POTASSIUM AND SODIUM
    GAMBLEJR, JL
    HACKENBR.CR
    [J]. FEDERATION PROCEEDINGS, 1969, 28 (02) : 283 - &
  • [7] Induction of permeability of the inner membrane of yeast mitochondria
    M. V. Kovaleva
    E. I. Sukhanova
    T. A. Trendeleva
    K. M. Popova
    M. V. Zylkova
    L. A. Uralskaya
    R. A. Zvyagilskaya
    [J]. Biochemistry (Moscow), 2010, 75 : 297 - 303
  • [8] Induction of permeability of the inner membrane of yeast mitochondria
    Kovaleva, M. V.
    Sukhanova, E. I.
    Trendeleva, T. A.
    Popova, K. M.
    Zylkova, M. V.
    Uralskaya, L. A.
    Zvyagilskaya, R. A.
    [J]. BIOCHEMISTRY-MOSCOW, 2010, 75 (03) : 297 - 303
  • [9] MITOCHONDRIAL FUNCTIONS IN ISCHEMIC MYOCARDIUM I - PROTON ELECTROCHEMICAL GRADIENT, INNER MEMBRANE-PERMEABILITY, CALCIUM-TRANSPORT AND OXIDATIVE-PHOSPHORYLATION IN ISOLATED-MITOCHONDRIA
    TOLEIKIS, A
    DZEJA, P
    PRASKEVICIUS, A
    JASAITIS, A
    [J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1979, 11 (01) : 57 - 76
  • [10] PHOTOSYNTHETIC MITOCHONDRIA - INTEGRATION OF A LIGHT-DRIVEN PROTON PUMP INTO MITOCHONDRIAL INNER MEMBRANE
    HOFFMANN, A
    HILDEBRANDT, V
    BULDT, G
    [J]. BIOPHYSICAL JOURNAL, 1993, 64 (02) : A132 - A132