Determination and Pharmacokinetics of Delsoline in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

The aim of this study is to develop an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to assess the concentration of delsoline in rat plasma and its pharmacokinetics. Twelve rats were randomly divided into two groups. One group was given delsoline (10 mg/kg) by oral administration, and the other was given delsoline (2 mg/kg) by intravenous administration. The blood (100 mu L) was obtained at 5 min, 0.25, 0.5, 1, 2, 4, 6, 8, 12, and 24 h after administration. After a simple protein precipitation procedure using methanol, the concentration of delsoline and the internal standard (diazepam) in rat plasma were detected by UPLC-MS/MS method. The precision, accuracy, recovery, matrix effect and the stability of the method were all in an acceptable range. The calibration plots were linear within the range of 1-2000 ng/mL, and the lower limit of quantification (LLOQ) of delsoline was 1 ng/mL. Pharmacokinetics of delsoline in rats after oral and intravenous administration were studied. The absolute bioavailability of delsoline was 5.2% in rats.