Interleukin-2 induces the activities of DNA topoisomerase I and DNA topoisomerase II in HuT 78 cells

被引：2
|
作者：
Pham, Minh-Hieu [1 ]
Kondapalli, Navya [1 ]
Reckord, Calvin L. [2 ]
Foglesong, P. David [1 ,2 ]
机构：
[1] Univ Incarnate Word, Dept Biol, UPO 311, San Antonio, TX 78209 USA
[2] Rutgers State Univ, Dept Biol, Camden, NJ 08102 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 390卷 / 03期
关键词：
DNA topoisomerase I; DNA topoisomerase II; Interleukin-2; GROWTH; PHOSPHORYLATION; LYMPHOCYTES; ACTIVATION; CHECKPOINT; INVITRO; PROTEIN; CANCER; ROLES; AP-1;
D O I：
10.1016/j.bbrc.2009.10.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The induction by interleukin-2 of DNA topoisomerase I and DNA topoisomerase 11 activities in the human T cell line HuT 78 was investigated. HuT 78 cells were treated with 1000 U of interleukin-2/ml, and extracts of the HuT 78 nuclei were prepared over a 24 h period. The extracts were assayed quantitatively for the activities of DNA topoisomerase I and DNA topoisomerase II. Three concomitant, transient increases of 3- to 11-fold in the specific activities of both DNA topoisomerase I and DNA topoisomerase 11 were observed following treatment with IL-2 at 0.5, 4, and 10 h after treatment with interleukin-2. The specific activities of both enzymes returned to base-line values after each of these transient increases. These results reveal that the activities of DNA topoisomerase I and DNA topoisomerase 11 are highly regulated in HUT 78 cells upon treatment with IL-2. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：577 / 580
页数：4
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