Forkhead transcription factor Foxo1 is essential for adipocyte differentiation

被引：64

作者：

Munekata, Keisuke ^{[1
]}

Sakamoto, Kazuichi ^{[1
]}

机构：

[1] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2009年 / 45卷 / 10期

关键词：

3T3-L1; Adipocyte; Adipogenesis; Differentiation; Foxo1; ACTIVATED RECEPTOR-GAMMA; MITOTIC CLONAL EXPANSION; PPAR-GAMMA; INSULIN SENSITIVITY; C/EBP-ALPHA; ADIPOGENESIS; FKHR; EXPRESSION; COMPLEX; PROTEIN;

D O I：

10.1007/s11626-009-9230-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We analyzed the physiological role of forkhead box class O 1 (Foxo1) in adipocyte differentiation by suppressing Foxo1 mRNA with siRNA specific for Foxo1. Mouse 3T3-L1 preadipocytes infected with an adenovirus expressing Foxo1-siRNA showed a marked decrease in lipid droplet formation when induced to differentiate into adipocytes. Adipocyte differentiation was most severely inhibited by exposing cells to Foxo1-siRNA before induction of differentiation. The incorporation of fluorescent-labeled glucose and fatty acid was significantly inhibited in cells deficient in Foxo1. RT-PCR revealed that downregulation of Foxo1 decreased the expression of the transcription factors, PPAR-gamma and C/EBP-alpha. By comparison, Foxo1-siRNA did not affect the expression of C/EBP-beta or C/EBP-delta during the early period of adipocyte differentiation. These results indicate that Foxo1 plays an essential role in adipocyte differentiation, especially at the very early stage of terminal adipocyte differentiation.

引用

页码：642 / 651

页数：10

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